三氧化二砷对A549细胞周期及cyclin D_1基因表达的影响  

Effects of arsenic trioxide on cell cycle of A549 cells and gene expression of cyclin D_1

作　　者：李晓霞[1] 郭红荣[1] 韦国桢[1] 曹琦[1] 

机构地区：[1]南京医科大学附属常州第二人民医院呼吸内科,江苏常州213003

出　　处：《海南医学院学报》2009年第12期1477-1480,1484,共5页Journal of Hainan Medical University

基　　金：常州市卫生局科技计划资助项目项目编号:(2004102)~~

摘　　要：目的:研究三氧化二砷(arsenic trioxide,As2O3)对肺腺癌A549细胞株细胞增殖、细胞周期以及cylin D1基因表达情况,初步探讨As2O3对肺腺癌细胞增殖的影响及其机制。方法:分别以不同浓度的As2O3作用于A549细胞,作用不同时间后,对细胞增殖活性、细胞周期及cylin D1基因表达的情况进行检测。结果:稍高浓度(>2μmol/L)的As2O3可抑制A549细胞增殖,并有剂量和时间依赖性,As2O3可抑制A549细胞由G1期进入S期。逆转录聚合酶链式反应(RT-PCR)检测cylin D1基因转录水平和细胞爬片免疫组化检测cyclin D1基因蛋白表达的相对强度,发现As2O3作用组cyclin D1基因表达与对照组相比明显减低(P<0.01)。结论:As2O3可有效抑制A549细胞增殖,抑制效应呈量效和时效关系,并且能阻碍细胞周期由G1进入S期,这一过程可能与As2O3抑制cyclin D1基因表达有关。Objective：To investigate the effects of arsenic trioxide （As2O3） on the proliferation,cycle of A549 cells and gene expression of cyclin D1 in lung adenocarcinoma and its mechanism. Methods： A549 cells were treated with different dosages of As2O3 for different periods of time,then its proliferation,cell cycle and the gene expression of cyclin D1 were detected and analyzed. Results： Being applied at the dosage of 〉2 μmol/L,As2 03 showed a dose and time dependent inhibition on the proliferation of A549 cells. Cell cycle arrest at G0/G1 in A549 cells can also be provoked by As203. The reverse transcriptase-polymerase chain reaction （RT-PCR） and immunohistochemistry detection revealed that the gene expression of cyclin D1 was significantly downregulated in As203 treated group（P 〈0.01 ）. Conclusion： As203can induce an dose and time dependent inhibition on the pro- liferation of A549 ceils. It can also provoke cell cycle arrest at G0/G1 stage which may be related with its function of depressing cyclin D1 gene expression.

关 键 词：肺腺癌 三氧化二砷 细胞周期 CYCLIN D1 

分 类 号：R734.2[医药卫生—肿瘤]