大肠肿瘤组织中人乳头状瘤病毒DNA的分子原位杂交研究  

Detection of human papillomavirus DNA in colorectal neoplasms by in situ hybridization with digoxin labelled DNA probes*

作　　者：黄培林[1,2] 陆伦根[1,2] 史志英[1,2] 尹克铮 张晓明[1,2] 

机构地区：[1]南京铁道医学院病理教研室 [2]扬州医学院病理教研室

出　　处：《华人消化杂志》1998年第9期771-773,共3页

基　　金：铁道部科技基金

摘　　要：目的探讨ＨＰＶ１６和ＨＰＶ１８感染与大肠肿瘤发生的关系．方法采用地高辛标记的ＨＰＶ１６和ＨＰＶ１８ＤＮＡ探针分别在４０例大肠癌和３０例大肠腺瘤组织石蜡切片上进行原位杂交探测ＨＰＶＤＮＡ．结果受检大肠腺瘤组织ＨＰＶＤＮＡ阳性８例（２７％），其中ＨＰＶ１６ＤＮＡ５例，ＨＰＶ１８ＤＮＡ３例，主要见于管状绒毛状腺瘤和绒毛状腺瘤；大肠腺癌组织中ＨＰＶＤＮＡ阳性１９例（４８％），其中ＨＰＶ１６ＤＮＡ１４例，ＨＰＶ１８ＤＮＡ５例．ＨＰＶＤＮＡ主要见于肿瘤细胞核中，少部分见于胞质中．研究表明，大肠癌组织中ＨＰＶ１６，１８ＤＮＡ检出阳性率明显高于大肠腺瘤；且大肠绒毛状腺瘤中ＨＰＶＤＮＡ阳性率明显高于管状腺瘤，阳性率接近高分化腺癌．结论ＨＰＶ１６。IM Abstract:To study the relationship between human papillomavirus (HPV) infection and the development of colorectal neoplasms.METHODS Thirty adenomas and 40 adenocarcinomas were analysed in this study by using in situ hybridization with digoxin labelled HPV16 and 18 DNA probes respectively.RESULTS HPV DNA was detected in 8 (27%) of 30 adenomas and in 19 (48%) of 40 adenocarcinomas. The expression of HPV DNA in adenomas and adenocarcinomas was significantly different (χ2=314,P<005). There was no correlation, however, between HPV and the location, Dukes' stage, differentiation of colorectal adenocarcinomas.CONCLUSION The presence and typing of HPV DNA in colorectal neoplasms may be studied by in situ hybridization and there is a close association between colorectal adenocarcinomas and HPV16, 18 infection.

关 键 词：病理学 遗传学 乳头 瘤病毒 大肠肿瘤 

分 类 号：R735.340.2[医药卫生—肿瘤]