多环芳烃降解菌的筛选及其对芘的降解研究  

Screening of PAH-degrading Strains and Study on Metabolic Properties of Pyrene

作　　者：王蕾[1] 聂麦茜[1] 王志盈[1] 玉亚[1] 徐会宁[1] 张睿[1] 

机构地区：[1]西安建筑科技大学环境与市政工程学院,陕西西安710055

出　　处：《环境科学与技术》2010年第1期43-47,共5页Environmental Science & Technology

基　　金：陕西省教育厅自然专项项目(07JK290)

摘　　要：以焦化厂排水沟底泥为菌源驯化筛选出6株多环芳烃降解菌。实验结果表明:各菌对芘均有一定的降解能力,不同菌体表面疏水性不同,这种不同可以影响到反应初期菌株对芘的表观降解率,菌体的疏水性表面较亲水性表面对芘有更强的吸附性;保存的一组天然混合菌对芘降解率较低,没有表现出优于单个菌株的协同作用;多环芳烃降解菌在芘培养液中生长快慢和降解能力没有必然联系。对两株菌体表面疏水性相差较大的菌株在不同条件下的芘降解性能研究结果表明:2#菌降解芘的最佳温度是30℃,9#菌降解芘的最佳温度是40℃;Mn2+对2#降解芘有促进作用,对9#菌几乎无影响,Cu2+对各菌芘降解均有不同程度的抑制作用;外加葡萄糖对于2#菌和9#菌的芘降解有促进作用。Six strains and a group of original mixed strains were isolated and screened from the bottom sludge of coke-plant wastewater. Results indicated that most of the bacteria had the best pyrene-degrading capabilities. Six strains had the different cell surface hydrophobicity, and the difference of cell surface properties could affect the pyrene removal rate in early reaction period. Results also showed that pyrene was more likely to be adsorbed onto cell surface with hydrophobicity than onto cell surface with hydrophobicity. The original mixed strains had the relatively low pyrene biodegradation rate, and showed no synergistic effect among different strains. The strain growth in pyrene cultural medium had no necessary connection with its pyrene biodegradation capability. Two strains with different cell surface properties were chosen to study the individual factors affecting pyrene biodegradation. Results showed that the optimum temperature to pyrene biodegradation was 30℃ for strain 2# and 40℃ for strain 9#. Mn^2＋ could promote the pyrene degradation velocity of strain 2# while it was almost no effect on strain 9#, Cu^2＋ had an inhibitory effect on pyrene biodegradation of the two strains. Exotic carbon source test showed that glucose was a beneficial substrate on the degradationof pyrene for both strain 2# and strain 9#.

关 键 词：芘 优势菌 降解 疏水性 温度 无机离子 外加碳源 

分 类 号：X132[环境科学与工程—环境科学]