拮抗放线菌BS-112的鉴定及抗菌物质粗提物对家蚕主要病原菌的抑制作用  

Identification of Actinomycete Strain BS-112 and Antagonistic Effects of its Crude Extract on Major Silkworm Pathogens

作　　者：张楠[1] 刘训理[2] 周启升[2] 曹宁宁[2] 张莎莎[1] 国辉[1] 

机构地区：[1]山东农业大学生命科学学院,山东泰安271018 [2]山东农业大学林学院,山东泰安271018

出　　处：《蚕业科学》2009年第4期796-802,共7页ACTA SERICOLOGICA SINICA

摘　　要：以绿僵菌(Nomuraea rileyi)和黑胸败血病菌(Bacillus sp.)为靶标,从泰山林地土壤中分离获得一株对多种家蚕病原菌具有强烈拮抗作用的放线菌BS-112。根据该菌株的形态特征、培养特征、理化性质、细胞壁组分及16S rDNA序列分析结果,将其鉴定为吸水链霉菌(Streptomyces hygroscopicus)。该菌株抗菌谱广,抗菌物质耐高温,在酸、碱环境比较稳定,抗菌物质粗提物对黑胸败血病菌和绿僵菌的最小抑菌浓度分别为19.53μg/mL和78.13μg/mL,最小杀菌浓度分别为78.13μg/mL和156.25μg/mL,表明在较低浓度下即可达到杀菌效果。Taking Nomuraea rileyi and Bacillus sp.as the target pathogens,an actinomyces strain BS-112 was isolated from soil in the forest land of Mountain Tai.This strain showed strong antagonistic activity against various silkworm pathogens.Based on its morphologic,culture,physiological and biochemical characteristics,and components of its cell wall as well as 16S rDNA sequence,BS-112 was identified as a Streptomyces hygroscopicus.It had a broad inhibition spectrum.The antifungal and antibacterial substances were resistant to high temperature and relatively stable under acidic or basic environment.The minimum inhibition concentration of extracellular crude antibacterial extracts against Bacillus sp.and N.rileyi was 19.53 and 78.13 μg/mL,respectively.And the minimum bactericidal concentration was 78.13 and 156.25 μg/mL,respectively.These results indicate that the obtained strain has satisfactory bactericidal effect under relatively low concen-tration.

关 键 词：拮抗放线菌BS-112 分类鉴定 抑菌活性 家蚕病原菌 

分 类 号：S884[农业科学—特种经济动物饲养]