The Enhancement of Osteogenesis by Scaffold Based on Mineralized Recombinant Human-like Collagen Loading with rhBMP-2  

作　　者：吴斌 郑启新 

机构地区：[1]Department of Orthopaedics,Union Hospital,Tongji Medical College,Huazhong University ofScience & Technology

出　　处：《Journal of Wuhan University of Technology(Materials Science)》2009年第6期956-960,共5页武汉理工大学学报（材料科学英文版）

基　　金：Funded by the National Natural Science Foundation of China(No.30170270);the National High-tech Research Program (863)(No. 2006AA320605)

摘　　要：A biomimetic scaffold based on mineralized recombinant collagen, nano-hydroxyapatite/recombinant human-like collagen/poly（lactic acid） （nHA/RHLC/PLA）, was prepared with recombinant human bone morphogenic protein-2 （rhBMP-2） for improving the osteoinductive property of the scaffold. The nHA/RHLC/PLA scaffolds loaded with 10 μg rhBMP-2 and the unloaded scaffolds were implanted subcutaneously in the rat model. The osteogenetic capacity of these composites was evaluated by CT scan, ALP activity test and histological observation at 4 and 8 weeks after implantation. The experimental results indicated that the osteogenic capability of the scaffolds loaded with rhBMP-2 was superior to the unloaded scaffold. It was concluded that rhBMP-2 can enhance the osteoinductive property of the nHA/RHLC/PLA scaffold and the nHA/RHLC/PLA scaffold loaded with rhBMP-2 have the good potential of being used in bone tissue engineering.A biomimetic scaffold based on mineralized recombinant collagen, nano-hydroxyapatite/recombinant human-like collagen/poly（lactic acid） （nHA/RHLC/PLA）, was prepared with recombinant human bone morphogenic protein-2 （rhBMP-2） for improving the osteoinductive property of the scaffold. The nHA/RHLC/PLA scaffolds loaded with 10 μg rhBMP-2 and the unloaded scaffolds were implanted subcutaneously in the rat model. The osteogenetic capacity of these composites was evaluated by CT scan, ALP activity test and histological observation at 4 and 8 weeks after implantation. The experimental results indicated that the osteogenic capability of the scaffolds loaded with rhBMP-2 was superior to the unloaded scaffold. It was concluded that rhBMP-2 can enhance the osteoinductive property of the nHA/RHLC/PLA scaffold and the nHA/RHLC/PLA scaffold loaded with rhBMP-2 have the good potential of being used in bone tissue engineering.

关 键 词：RHBMP-2 bone tissue engineering OSTEOGENESIS biomaterial 

分 类 号：R318.0[医药卫生—生物医学工程]