PFGE分型技术在3起沙门菌食物中毒调查中的应用  

Investigation of Salmonella food poisoning using pulsed-field gel electrophoresis

作　　者：梅玲玲[1] 罗芸[1] 叶菊莲[1] 朱敏[1] 潘雪霞[1] 占利[1] 

机构地区：[1]浙江省疾病预防控制中心,杭州310051

出　　处：《中国卫生检验杂志》2009年第12期2954-2956,共3页Chinese Journal of Health Laboratory Technology

摘　　要：目的：应用PFGE分型技术,对3起沙门菌食物中毒事件分离株进行基因分型和溯源,为沙门菌溯源提供依据。方法：用限制性内切酶Xbal切割,用PFGE技术建立PFGE基因图谱,采用Band based/Dice方法计算PFGE基因图谱的相似性系数（F值）。结果：同一起食物中毒事件中分离的沙门菌PFGE基因图谱基本相同,经Band based/Dice方法计算,相似性系数（F值）大于等于0.96;蛋糕店和某学院食物中毒事件中分离的沙门菌PFGE基因图谱仅相差1～2条DNA条带。结论：奶油均是引起蛋糕店食物中毒食品,北京鸡肉卷为引起绍兴某学院食物中毒食品;蛋糕店和某学院食物中毒事件分离的沙门菌同源性很高,来源可能相同。PFGE分型方法稳定可靠,溯源效果好,对探明沙门菌食物中毒事件污染源作用显著。Objective：To apply pulsed-field gel electrophoresis（PFGE）to investigating trace infection sources and genotype of Salmonella strains isolated from food poisoning.Methods：Salmonella strains were analyzed by pulsed-field gel electrophoresis with restriction enzyme Xbal.The similarity coefficient of PFGE patterns were calculated using Band based/Dice assay.Results：PFGE patterns of strains from the same outbreak of food poisoning were identical.Through Band based/Dice assay the similarity coefficient is more than or equal to 0.96.The PFGE pattern of strain isolated from cake store was different from that of strain isolated from certain institute.Conclusion：The food poisoning is induced by the cutting tool and butter in cake shop.Then the food poisoning was induced by Beijing chicken roll in a certain institute.The above results indicated that two strains isolated from two institutes may be having the same source.PFGE is a stabile,reliable assay having high resolution ability for trace infection sources.PFGE plays important roles for investigating trace infection sources in Salmonella food poisoning.

关 键 词：沙门菌食物中毒 脉冲场凝胶电泳 

分 类 号：R155.31[医药卫生—营养与食品卫生学]