双水相技术在萃取橙皮苷中的应用  

Aqueous Two-phase Extraction of Hesperidin

作　　者：文赤夫[1] 赵凯[1] 康练常[1] 汪兰[1] 

机构地区：[1]吉首大学生物资源与环境科学学院,湖南吉首416000

出　　处：《食品科学》2009年第22期71-73,共3页Food Science

基　　金：湖南省教育厅项目(07C529)

摘　　要：研究双水相萃取橙皮苷的成相体系及影响萃取率的因素。通过试验研究选择丙酮与(NH4)2SO4-H2O为双水相体系,研究盐用量、pH值及粗提物的用量对萃取率的影响。结果表明:在90%丙酮与(NH4)2SO4溶液体积各为10ml的情况下,(NH4)2SO4用量4.0g、样品0.35g、pH4的条件下,萃取效率最佳。经萃取,橘皮中的橙皮苷主要进入有机相中,萃取率为98.22%。通过薄层分析,在365nm紫外灯下,上相溶液与标准品的斑点Rf值接近,下相溶液检测不到橙皮苷。An aqueous two-phase system consisting of acetone,water and ammonium sulfate was developed for the purification of crude hesperidin extracted from orange peel using aqueous sodium carbonate solution.Effects of amount of ammonium sulfate addition,amount of crude hesperidin and pH on hesperidin extraction were studied.Results showed that an optimum extraction rate of hesperidin as high as 98.22% was obtained when the aqueous two-phase system consisting of 10 ml of 90% aqueous acetone and 10 ml of water containing 4.0 g of ammonium sulfate at pH 4 was used for treating 0.35 g of crude hesperidin.Thin layer chromatographic（TLC） analysis indicated that hesperidin was mainly partitioned into acetone phase with a Rf value close to that of hesperidin standard.

关 键 词：双水相萃取体系 橙皮苷 紫外分光光度法 橘皮 薄层分析法 

分 类 号：Q664.3[生物学—生物物理学]