阵发性睡眠性血红蛋白尿症患者T淋巴细胞对造血祖细胞的影响  

Effect of T lymphocytes on hematopoietic progenitors in patients with paroxysmal nocturnal hemoglobinuria

作　　者：高清平[1] 夏虹[1] 陈友华[1] 李秀珍[1] 陈济民[1] 

机构地区：[1]湖北医科大学附属第一医院血液科

出　　处：《中华血液学杂志》1998年第10期522-524,共3页Chinese Journal of Hematology

摘　　要：目的：研究阵发性睡眠性血红蛋白尿症（ＰＮＨ）患者的Ｔ淋巴细胞对造血祖细胞的影响。方法：将ＰＮＨ患者外周血制备的植物血凝素刺激的Ｔ淋巴细胞条件培养液（ＰＨＡＴＣＭ）加入造血祖细胞的培养基中，用于ＰＮＨ患者和正常人骨髓的粒巨噬细胞系集落形成单位（ＣＦＵＧＭ）和红系爆式集落形成单位（ＢＦＵＥ）的培养。结果：在含有初发ＰＮＨ患者的ＰＨＡＴＣＭ培养条件下，ＰＮＨ患者和正常人骨髓ＣＦＵＧＭ和ＢＦＵＥ数明显低于含有正常人ＰＨＡＴＣＭ培养条件下的集落数；在含有好转和治愈的ＰＮＨ患者的ＰＨＡＴＣＭ培养条件下，正常人骨髓ＣＦＵＧＭ和ＢＦＵＥ数增加和恢复到正常范围。结论：初发ＰＮＨ患者的Ｔ淋巴细胞对造血祖细胞的支持作用减弱。Objective:To study the effect of T lymphocytes on hematopoietic progenitors in patients with paroxysmal nocturnal hemoglobinuria(PNH).Methods:Phytohemagglutinin stimulating T lymphocytes conditioned medium(PHA TCM) prepared from PNH(PHA TCM PNH) and normal blood(PHA TCM N) was added to the cultures of PNH or normal CFU GM and BFU E.Results:In cultures containing PHA TCM PNH prepared from newly diagnosed PNH patients,the yields of CFU GM and BFU E from either PNH or normal bone marrow were reduced more than they did in cultures containing PHA TCM N;but in cultures containing PHA TCM PNH prepared from PNH patients in remission,they were normal.Conclusion:T lymphocytes from newly diagnosed PNH patients exerts an inhibitory effect on the in vitro growth of CFU GM and BFU E.

关 键 词：血红蛋白尿症 PNH 造血干细胞 T细胞 

分 类 号：R556.64[医药卫生—血液循环系统疾病]