香菇多糖诱导人肺腺上皮细胞β-防御素-2基因表达的研究  

Lentinan Induces the Expression of Human-β-Defensin-2 in Pulmonary Epithelial Cells

作　　者：周焱[1] 董碧蓉[1] 滕丽花[1] 王慧[1] 

机构地区：[1]四川大学华西医院老年科,成都610041

出　　处：《生物医学工程学杂志》2009年第6期1311-1314,共4页Journal of Biomedical Engineering

摘　　要：探讨香菇多糖诱导人肺腺上皮细胞的人-β-防御素-2(HBD-2)基因表达的情况。香菇多糖体外刺激人肺腺上皮细胞(SPC-A-1)后,应用RT-PCR技术检测SPC-A-1细胞HBD-2 mRNA的表达。香菇多糖可上调人肺腺上皮细胞(SPC-A-1)HBD-2 mRNA的表达;这种增强表达与药物的刺激剂量和刺激时间具有一定的相关性。To determine whether lentinan could upregulate the expression of human-β-defensin-2（HBD-2） in pulmonary epithelial cells（SPC-A-1）, we stimulated pulmonary epithelial cells with lentinan and detected the expression of HBD-2mRNA by RT-PCR test. The results demonstrated that the expression of HBD-3mRNA in SPC-A-1 could be induced by lentinan in a concentration and time-dependent manner.

关 键 词：香菇多糖 人-β-防御素-2人肺腺上皮细胞 基因诱导表达 

分 类 号：R151[医药卫生—营养与食品卫生学]