北京早园竹叶活性成分的分离与鉴定  

Isolation and identification of activated substances of North Ph. praecox leaves

作　　者：祖桂芳[1] 彭辉[2] 赵晓红[2] 

机构地区：[1]首都师范大学生命科学学院,北京100037 [2]北京联合大学应用文理学院,北京100083

出　　处：《食品工业科技》2009年第12期123-125,129,共4页Science and Technology of Food Industry

基　　金：北京市自然科学基金项目(7092015)

摘　　要：目的:对北京早园竹中的活性成分进行提取、分离和鉴定。方法:用80%乙醇-水溶液超声辅助提取法提取早园竹叶活性物质,并用SP825大孔树脂对提取物进行分离。采用不同浓度的乙醇水溶液洗脱得到不同的洗脱成分,用高效液相法对总提取物和不同洗脱成分进行检测。结果:在总提取物中检测到C-黄酮苷类和酚酸类两类生物活性的物质,含量分别为0·31%和0·84%;经SP825大孔树脂纯化后,在10%乙醇洗脱成分酚酸类物质含量达到5%,在30%乙醇洗脱成分中C-黄酮苷类物质含量达到12·8%。结论:80%乙醇-水溶液超声辅助提取法能有效提取出早园竹叶活性成分,SP825大孔树脂能对提取物进行进一步的分离和纯化。Objective： To extract and identify activated substances from North Ph. praecox leaves. Methods： The activated substances of North Ph.praecox leaves were extracted by ethanol-aqueous solution with ultrasonicassisted extraction, and the extracts were further purified and isolated by SP825 resin column chromatograph. C-glycosides and phenolic acids in the extracts were analyzed qualitatively, and the contents of C-glycosides and phenolic acids in different eluent were quantified by detective high-performance liquid chromatography （HPLC）. Results：C-glycosides and phenolic acids were detected in crude extracts, and the proportion of C-glycosides is 0.84%, phenolic acids is 0.31%.After purifying by SP825 resin column chromatograph,the content of C-glycosides in 30% ethanol eluent was 12.8%, one of phenolic acids in 10% ethanol eluent was 5% .Conclusion： Ethanol- aqueous solution with ultrasonic-assisted extraction can extract activated substances from North Ph. praecox leaves effectively,and SP825 resin column chromatograph can further isolate different activated substances.

关 键 词：早园竹叶 C-黄酮苷 酚酸 

分 类 号：TS201.2[轻工技术与工程—食品科学]