Notch1和表皮生长因子受体在舌鳞癌及癌前病变中的表达  

Expression of Notch1 and epidermal growth factor receptor in human tongue squamous cell carcinoma and precancerous lesion

作　　者：黄红杰[1] 平飞云[2] 胡济安[3] 赵士芳[4] 

机构地区：[1]杭州师范大学附属医院口腔科,浙江杭州310015 [2]浙江大学医学院附属第二医院口腔科,浙江杭州310008 [3]浙江大学医学院附属口腔医院口腔病理科,浙江杭州310006 [4]浙江大学医学院附属口腔医院口腔颌面外科,浙江杭州310006

出　　处：《华西口腔医学杂志》2009年第6期665-668,672,共5页West China Journal of Stomatology

基　　金：浙江省自然科学基金资助项目(Y206192);浙江省教育厅科研基金资助项目(20061057)

摘　　要：目的研究舌鳞癌(TSCC)及癌前病变中Notch1的表达,探讨其与表皮生长因子受体(EGFR)之间的潜在关系。方法免疫组化检测41例人TSCC、39例舌黏膜白斑(LP)和7例正常舌黏膜标本中Notch1和EGFR的表达。结果在正常舌黏膜和LP中,Notch1阳性表达主要位于角化层,部分颗粒层和棘层细胞也有表达,基底层无表达;而EGFR阳性表达主要位于基底层,棘层少见表达,颗粒层和角化层无表达。在TSCC中,Notch1阳性表达多见于癌巢中鳞状化生的角化细胞及类棘层细胞,周边细胞无表达;而EGFR阳性表达主要位于癌巢周边细胞,鳞状化生的角化细胞及类棘层细胞无表达。结论Notch1在舌黏膜上皮中促进细胞分化,在TSCC中起抑癌作用;而EGFR的表达可能抑制Notch1的表达,以维持细胞增殖,并促进TSCC发生发展。Objective To investigate the expression of Notch1 in human tongue squamous carcinoma（TSCC） and precancerous lesion, and to explore the potential relation between Notchl and epidermal growth factor receptor （EGFR）. Methods The expression of Notch1 and EGFR was detected in human TSCC （n=41）, tongue leukoplakia （LP） （n=39） and normal tongue mucosa（n=7） by immunohistochemistry. Results In normal tongue mucosa and LP, the positive staining of Notch1 was mainly distributed in stratum corneum, partially in stratum granulosum and stratum spinosum, but not in stratum basale, while the positive staining of EGFR was mainly distributed in stratum basale, rarely in stratum spinosum, but not in stratum granulosum and stratum comeum. In TSCC, Notch1 expression was mainly distributed in locations of squamous metaplasia, but not in peripheral cells of carcinomas, while EGFR expression was detected mainly in peripheral cells of carcinomas, but not in locations of squamous metaplasia. Conclusion Notchl promotes the differentiation of epithelial cells in tongue mucosa and acts as a tumor suppressor in TSCC. EGFR may act as a negative regulator of Notch1 expression in epithelium of tongue mucosa and TSCC, for maintaining cell proliferation and promoting the tumorigenesis and progression of TSCC.

关 键 词：舌鳞状细胞癌 癌前病变 白斑 NOTCH1 表皮生长因子受体 

分 类 号：R739.8[医药卫生—肿瘤]