一种Vc二步发酵伴生菌的初筛方法  

A Preliminary Screening Method of Associated Bacteria in Two-stage Fermentation of Vitamic C

作　　者：杨伟超[1] 汲涌 荆士华 张朝印 姜铭妍 谌明 徐慧[1] 

机构地区：[1]中国科学院沈阳应用生态研究所,辽宁沈阳110016 [2]东北制药总厂,辽宁沈阳110026

出　　处：《生物技术》2009年第6期51-53,共3页Biotechnology

摘　　要：目的:建立一种Vc二步发酵高效伴生菌的快速初筛方法。方法:通过测定OD650,以伴生菌进入对数期的早晚和对数期持续长短为判断菌株伴生潜力的依据。结果:10h前进入对数期,对数期持续时间约12h的菌株具有高效的促进小菌生长和发酵产古龙酸的潜力。概率为71%。结论:以伴生菌进入对数期的早晚和对数期持续长短为判断依据的新的菌株快速初筛方法,简便、高效,利于大规模初筛应用。Objective： A new preliminary screening method of associated bacteria in two-stage fermentation of vitamin C was established in this article.Method： the judgment basis was,by determining OD650 of bacteria,that the time when bacteria entered into log phase and how long the log phase sustained.Result：The screened bacteria whose log phase entered in 10 hours and sustained for 12 hours had a potential to promote the growth of Gluconobacter oxydans,and improve its ability to product L-gulonic acid.the probability was 71 percent.Conclusion：The new preliminary screening method,on the basis of time when bacteria entered into log phase and how long the log phase sustained,was proved effective and easy to operate.

关 键 词：2-酮基-L-古龙酸 VC二步发酵 伴生菌 初筛 

分 类 号：Q93-331[生物学—微生物学]