广州地区石蜡包埋宫颈癌组织中人乳头瘤病毒的检测与分型  

Identification and genotyping of oncogenic type of human papillomavirus in paraffin-embedded cervical cancer samples in Guangzhou

作　　者：曾莉[1] 俞守义[1] 郭遂群[2] 云径平[3] 章俊[4] 

机构地区：[1]南方医科大学公共卫生与热带医学学院,广东广州510515 [2]南方医科大学南方医院妇产科,广东广州510515 [3]中山大学附属肿瘤医院病理科,广东广州510060 [4]南方医科大学珠江医院肾内科,广东广州510282

出　　处：《南方医科大学学报》2009年第12期2485-2487,共3页Journal of Southern Medical University

基　　金：广东省医学科研基金(A2007361);广东省科技计划项目(2009B030801218)

摘　　要：目的调查近30年广州地区宫颈癌组织中人乳头瘤病毒(Human papillomavirus,HPV)的感染状况和HPV主要型别。方法采用HPV通用引物对石蜡包埋宫颈癌组织标本中的HPVL1区基因序列进行PCR扩增,根据PCR产物测序所得序列分析对HPV分型。结果99例宫颈癌组织HPVDNA检出率为26.2%,广州宫颈癌患者存在HPV16、18、33、52和58五种型别。结论在本地区的99例宫颈癌病理组织中,以16、58和33基因型最为常见,共占HPV阳性型别构成比的88.4%,说明广州地区与宫颈癌密切相关的HPV基因型别较为集中。Objective To investigate human papillomavirus （HPV） infection and genotyping in patients with cervical cancer in Guangzhou in the last 3 decades. Methods HPV L1 gene fragment in paraffin-embedded cervical cancer samples was amplified by HPV-specific PCR with consensus primers, and typing of HPV strains was performed on the basis of sequence analysis of the PCR products. Results The positivity rates of HPV DNA was 26.2% in the 99 cases of cervical cancer. Five HPV genotypes were identified including HPV16, 18, 33, 52 and 58. Conclusion HPV16, 58 and 33 are the most common genotypes of HPV, accounting for over 88.4% in the total infected cases, suggesting that the HPV genotypes closely related to cervical cancer is more centralized in Guangzhou.

关 键 词：人类乳头瘤病毒 宫颈癌 聚合酶链反应 测序分析 

分 类 号：R737.33[医药卫生—肿瘤]