iNOS、MMP-2及Notch1在胃癌中的表达与转移的关系  

Expression of iNOS,MMP-2 and Notch1 in gastric cancer and their correlation with metastasis

作　　者：黄培新[1] 王俊珊[1] 陶春华[1] 黄志刚[1] 刘占举[1] 

机构地区：[1]同济大学附属第十人民医院消化内科,上海200072

出　　处：《同济大学学报（医学版）》2009年第6期53-57,共5页Journal of Tongji University(Medical Science)

基　　金：教育部新世纪优秀人才支持计划基金(NECT-05-0609)

摘　　要：目的研究iNOS、MMP-2及Notch1蛋白在胃癌组织中的表达及其与转移的关系。方法取68例胃癌组织切片,采用免疫组化染色法检测iNOS,MMP-2及Notch1在胃癌组织中的表达,并统计分析其与胃癌临床病理学特征的关系,分析三种蛋白的表达与胃癌转移的关系。结果iNOS的阳性表达与淋巴转移、浸润深度、临床分期相关(P<0.05)。并随浸润深度的增加,其表达逐渐增强(P<0.05),淋巴结转移阳性者iNOS的表达高于阴性者(P<0.05)。MMP-2蛋白阳性表达率与淋巴转移、浸润程度及临床分期相关(P<0.05)。Notch1蛋白阳性表达率与肿瘤大小、分化程度、淋巴结转移和浸润程度的相关性均有统计学意义(P<0.05)。68例胃癌患者中,发生淋巴结转移者共39例,其中iNOS及MMP-2的表达分别与Notch1表达呈显著正相关(P<0.05)。而MMP-2与iNOS的表达并无相关趋势(γ=0.015,P>0.05)。结论iNOS、MMP-2及Notch1三种蛋白共同参与了胃癌的发生发展,且iNOS及MMP-2可能通过共同的Notch1信号途径,促进肿瘤血管生成而促进胃癌转移。Objective To investigate the expression of inducible nitric oxide synthase （iNOS）, matrixmetalloproteinase-2 （MMP-2） and Notch1 in gastric cancer patients and their correlation with metastasis. Methods The expressions of iNOS, MMP-2 and Notchl were measured with respective immunohistochemical method in the tissues from 67 patients with gastric cancers. The correlation of three selected proteins to the clinical pathological features of the patients and metastasis of gastric cancer were analyzed statistically. Results The expression of iNOS in gastric cancer patients correlated with lymphnode metastasis, invasive extent and TNM stages （ P 〈 0.05 ）, and its expression increased with the escalation of invasion extent （P 〈 0.05 ）, and was found higher in the metastasis patients than in non-metastasis patients （P 〈 0.05 ）. The expression of MMP-2 had significant correlation with lymphnode metastasis, invasion extent and TNM stages, while the expression of Notchl had significant correlation with the tumor size, differentiation level, lymphnode metastasis, invasion extent and TNM stages （P 〈 0.05 ）. Lymphnode metastasis was found in 39 patients in all patients investigated. The expression of iNOS and MMP-2 positively correlated with the expression of Notchl, respectively （ P 〈 0.05 ）, but had no significant correlation with each other （γ = 0.015, P 〉 0.05 ）. Conclusion iNOS, MMP-2 and Notchl all participate in the progress of gastric cancer. Moreover, iNOS and MMP-2 may both act through the Notchl signaling pathway and eventually can regulate the tumor angiogenesis and promote metastasis of gastric cancer.

关 键 词：胃肿瘤 基质金属蛋白酶-2 诱导型一氧化氮合酶 NOTCH1 转移 

分 类 号：R735.2[医药卫生—肿瘤]