凹叶厚朴中具有α-葡萄糖苷酶抑制活性的成分  

Constituents with Inhibitory Activity against α-glycosidase from Magnolia offcinalis Rehder et Wils

作　　者：瞿庆喜[1] 朱庆亚[2] 喻凯[2] 王明奎[1] 

机构地区：[1]中国科学院成都生物研究所生态恢复重点实验室,成都610041 [2]西南交通大学生命科学与工程学院,成都610013

出　　处：《应用与环境生物学报》2009年第6期796-798,共3页Chinese Journal of Applied and Environmental Biology

基　　金：四川省应用基础研究项目(No.2007J13-059)资助~~

摘　　要：凹叶厚朴是四川省人工栽种的一种用途广泛的常用中药.在发现凹叶厚朴乙醇提取物具有较强的α-葡萄糖苷酶抑制活性基础上,通过活性跟踪,对该提取物进行了分离,发现其中的生物碱显示了较强的抑制活性.通过溶剂萃取、树脂吸附和反复硅胶柱层析等分离方法从凹叶厚朴乙醇提取物中分离得到6个生物碱,用质谱和核磁共振等波谱方法分别鉴定为木兰箭毒碱,木兰花碱,鹅掌楸碱,蕃荔枝碱,罗默碱和Lysicamine,并应用小肠α-葡萄糖苷酶模型测定了它们对α-葡萄糖苷酶的抑制作用.其中,番荔枝碱和木兰箭毒碱的抑制活性较好,分别为60%和62%;其它4个生物碱成分活性几乎相当,鹅掌楸碱为46%,罗默碱为51%,Lysicamine为49%,木兰花碱为51%.Magnolia officinalis Rehd. et Wils,an extensively cultivated traditional Chinese medicine in Sichuan,China,was widely used for curing various diseases. So far,there is no report about the inhibitory activity of this plant against α-glycosidase. The ethanol extract from M. officinalis showed a good inhibitory activity against α-glycosidase. The bio-guided separation showed that the alkaloids were the active components. By solvent extraction,resin absorption and repeated silica gel column chromatography,six known alkaloids as glycosidase inhibitor were isolated from M. officinalis and they were identified as liriodenine （1）, anonaine （2）, roemerme （3）, lyslcamine （4）, magnoflorine （5） and magnocurarine （6） by spectroscopic methods. The inhibitory activity against ct-glycosidase was determined in alvine screening model. Among them, anonaine and magnocurarine had better inhibiting activities （inhibition rates of 60% and 62%） than the other four alkaloids （from 46% to 51%）. Fig 2, Ref 10

关 键 词：凹叶厚朴 生物碱 Α-葡萄糖苷酶 抑制活性 

分 类 号：R284[医药卫生—中药学]