葡萄籽原花青素的纯化与结构研究  

Purification and Structural Characterization of Proanthocyanidin from Grape Seed

作　　者：李春阳[1] 张红城[2] 王乃富[1] 刘文旭[1] 

机构地区：[1]江苏省农业科学院农产品加工研究所,江苏南京210014 [2]中国农业科学院蜜蜂研究所,北京100093

出　　处：《食品科学》2009年第23期218-223,共6页Food Science

基　　金：2006年度江苏省科技攻关(农业;苏北星火带)项目(BE2006313)

摘　　要：将大孔树脂HP-2MGL和交联葡聚糖凝胶SephadexLH-20用于葡萄籽原花青素的纯化。HP-2MGL可除去蛋白、多糖、高聚物等杂质,获得原花青素含量为96.5%的GSPP3。SephadexLH-20能够进一步纯化GSPP3,获得低聚原花青素GSPP3-SP1和GSPP3-SP2。经HPLC柱分离,GSPP3-SP1有4个峰,GSPP3-SP2有7个峰。GSPP3-SP1和GSPP3-SP2质谱分析发现,含有大量m/z287、m/z289、m/z290、m/z408、m/z426、m/z577、m/z578、m/z580等分子离子及碎片质谱信息,证明是由儿茶素和表儿茶素构成的原花青素二聚体的同分异构体。激光光散射与GPC联用测定原花青素粗提物GSP的重均相对分子质量为4.15×104,多分散系数为1.65;GSPP3的重均相对分子质量为4.26×103,平均聚合度为13,多分散系数为1.56,相对分子质量分布曲线呈对称分布,以高聚合度原花青素为主;葡萄籽原花青素纯化分级级分GSPP3-SP的重均相对分子质量为1.89×103,平均聚合度为6,多分散系数为1.45,以低聚合度原花青素为主。Macroporous resin HP-2MGL and SephadexLH-20 Gel was used to purify the proanthocyanidin from grape seed. Fraction GSPP3HP-2MGL was obtained by separation of crude grape seed extract through HP-2MGL and its proanthocyanidin content reached 96.5%. The fraction was further purified with SephadexLH-20 and two proanthocyanidin dipolymers GSPP3-SP1 and GSPP3-SP2 were resulted. HPLC analysis showed 4 peaks from GSPP3-SP1 and 7 peaks from GSPP3-SP2 were recognized. Mass spectrums revealed the abundance of the fragment ions like m/z 287, m/z 289, m/z 290, m/z 408, m/z 426, m/z 577, m/z 578 and m/z 580, suggesting this proanthocyanidin was a catechuic acid epigallocatechin--epicatechin dimer. The MW of GSP was determined to be 4.15 × 104 by laser light scattering combined with GPC and the coefficient of polydispersity was 1.65. The MW of GSPP3 was 4.26×103 with the average degree of polymerization of 13 and the coefficient of polydispersity of 1.56. The relative molecular weight curve showed a symmetrical distribution and this proanthocyanidin was highly polymerized. GSPP3-SP was found to lowly polymerized proanthocyanidin with the MW of 1.89×103, the average degree of polymerization of 6 and the coefficient of polydispersity of 1.45.

关 键 词：葡萄籽 原花青素 纯化 结构 

分 类 号：S663.1[农业科学—果树学]