八种蜂花粉中脂肪酸的测定及GC-MS分析  

GC-MS Analysis and Determination of Fatty Acids in Bee Pollen

作　　者：张红城[1] 董捷[1] 曲臣[2] 任向楠[1] 

机构地区：[1]中国农业科学院蜜蜂研究所,北京100093 [2]东北农业大学生命科学学院,黑龙江哈尔滨150001

出　　处：《食品科学》2009年第24期419-424,共6页Food Science

基　　金：国家公益性行业(农业)科研专项经费项目(nyhyzx07-041);国家蜂产业技术体系资助项目(NYCYTX-43)

摘　　要：采用索氏提取法对茶花花粉、罂粟花粉、桃花花粉、莲花花粉、黄柏花粉、籽瓜花粉、蒲公英花粉、蚕豆花粉中的粗脂肪进行提取,测定其粗脂肪和游离脂肪酸含量。对粗提物进行甲基衍生化处理,用气相色谱-质谱联用技术进行分离鉴定,测定脂肪酸的相对含量。结果显示八种蜂花粉均含有丰富的脂肪酸,是重要的功能因子。The crude fat was extracted from 8 kinds of bee pollen such as camellia pollen, poppy pollen, peach pollen, lotus flower pollen, amboyna pollen, watermelon pollen, dandelion pollen, broad beans pollen through Soxhlet extraction. The contents of crude fat and free fatty acids in bee pollen were also determined. The relative contents of fatty acids were further analyzed and determined via GC-MS after esterification of crude extracts. Results indicated that 8 kinds of bee pollen had plentiful fatty acids, which were important function factors for nutrition.

关 键 词：蜂花粉 脂肪酸 气相色谱-质谱 

分 类 号：S896.4[农业科学—特种经济动物饲养]