酵母细胞表面展示技术及其在非水相酶催化合成中的应用  

Yeast cell surface display and its application of enzymatic synthesis in non-aqueous phase

作　　者：韩双艳[1] 李华珍[1] 金子[1] 黄登峰[1] 任昌琼[1] 林影[1] 

机构地区：[1]华南理工大学生物科学与工程学院,广州510006

出　　处：《生物工程学报》2009年第12期1784-1788,共5页Chinese Journal of Biotechnology

基　　金：广东省-国家自然科学基金联合基金(No.U0773001)资助~~

摘　　要：酵母展示技术是将外源蛋白与酵母细胞壁蛋白融合,并将外源蛋白表达在酵母细胞表面。酵母展示技术已广泛应用于各种功能蛋白的表达及筛选。以下重点介绍酵母展示技术在脂肪酶展示体系构建及其在脂肪酸甲酯、短链芳香酯及糖酯生物合成中的应用。Yeast surface display involves that the exogenous protein,which was fused with the yeast outer shell cell wall protein,was genetically anchored on the yeast cell surface.It has been widely used in expression and screening of functional protein.Here,we focused on the construction of lipase-displaying systems and its application in enzymatic biosynthesis,such as fatty acid methyl esters,short-chain flavour esters and sugar esters applications,and so on.

关 键 词：酵母展示 全细胞催化 甲酯 芳香酯 糖酯 

分 类 号：Q814[生物学—生物工程]