High expression of osteoglycin decreases gelatinase activity of murine hepatocarcinoma Hca-F cells  

作　　者：Xiao-Nan Cui Jian-Wu Tang Bo Song BO Wang Shan-Yan Chen Li Hou 

机构地区：[1]Department of Oncology, the 1 st Affiliated Hospital of Dalian Medical University, Dalian 116011, Liaoning Province, China [2]Department of Pathology, Dalian Medical University, Dalian 116044, Liaoning Province, China [3]Tongji Medical College of Huazhong University of Science & Technology, Wuhan 430074, Hubei Province, China

出　　处：《World Journal of Gastroenterology》2009年第48期6117-6122,共6页世界胃肠病学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China,No.30500586

摘　　要：AIM:To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells.METHODS:A eukaryotic expression plasmid pIRE Spuro3 osteoglycin(+)was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node,liver,spleen or in DMEM medium.The activity of gelatinases was examined through zymographic analysis.RESULTS:High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node,and at the same time,decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo.CONCLUSION:High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node;regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression.AIM： To investigate the possible correlation between osteoglycin expression and gelatinase activity of mouse hepatocarcinoma Hca-F cells. METHODS： A eukaryotic expression plasmid pIRESpuro3 osteoglycin（＋） was constructed and transfected into Hca-F cells to investigate the possible correlation between osteoglycin expression and gelatinase activity of Hca-F cells cultured with extract of lymph node, liver, spleen or in DMEM medium. The activity of gelatinases was examined through zymographic analysis. RESULTS： High expression of osteoglycin attenuated the gelatinase activity of Hca-F cells cultured with extract of lymph node, and at the same time, decreased the metastatic potential of Hca-F cells to peripheral lymph nodes in vivo. CONCLUSION： High expression of osteoglycin decreases the gelatinase activity of Hca-F cells cultured with extract of lymph node; regulation of gelatinase activity might be one of mechanisms that osteoglycin contributes to lymphatic metastasis suppression.

关 键 词：OSTEOGLYCIN TRANSFECTION Hepatocellular carcinoma Neoplasm metastasis Genes GELATINASES 

分 类 号：Q492.5[生物学—生理学] TS41[农业科学—烟草工业]