靶向mdr1不同位点的siRNA对两种耐药细胞MDR的逆转效果  

Reversal effects of siRNAs targeting different regions of the mdr1 gene on multidrug resistance in two drug-resistant tumor cell lines

作　　者：张敏[1] 李勇莉[1] 高建凯[1] 王国栋[1] 高福莲[1] 

机构地区：[1]新乡医学院组织胚胎学教研室,河南省新乡市453003

出　　处：《世界华人消化杂志》2009年第33期3387-3393,共7页World Chinese Journal of Digestology

基　　金：河南省科技攻关计划基金资助项目;No.072102310073~~

摘　　要：目的:探讨靶向mdr14个不同位点的siRNAs对胃癌耐药细胞SGC7901/VCR和人红白血病耐药细胞K562/A02的作用效果.方法:设计并体外转录合成4条靶向mdr1的siRNAs(mdr1si326、mdr1si1513、mdr1si2631及mdr1si3071),分别转染SGC7901/VCR细胞和K562/A02细胞,用RT-PCR和免疫组织化学检测mdr1mRNA与P-gp的表达,流式细胞仪检测细胞内阿霉素(ADR)的蓄积,MTT法检测细胞对阿霉素的敏感性.结果:4条siRNAs对人胃癌细胞SGC7901/VCRmdr1介导的MDR逆转效果由高到低依次为mdr1si326、mdr1si2631、mdr1si3071及mdr1si1513;对人红白血病细胞K562/A02mdr1介导的MDR逆转效果由高到低依次为mdr1si326、mdr1si2631、mdr1si3071及mdr1si1513.结论:4条siRNAs对SGC7901/VCR和K562/A02两种耐药细胞作用效果趋势相似.AIM： To investigate the effects of four siRNAs targeting different regions of the multidrug resistance 1 （mdrl gene） on multidrug resistance （MDR） of SGC7901/VCR and K562/ A02 cells. METHODS： Four siRNAs （mdrlsi326, mdrlsi1513, mdrlsi2631 and mdrlsi3071） target- ing the mdrl gene were designed and transfected into SGC7901/VCR and K562/A02 cells, respectively. The expression level of mdrl mRNA was analyzed by reverse transcription-polymerase chain reaction （RT-PCR）. The expression level of P-glycoprotein （P-gp） was detected by immunohistochemistry. The accumulation of intracellular adriamycin （ADR） was examined by flow cytometry. The cell sensitivity to ADR was detected by methyl thiazolyl tetrazolium （MTT） assay. RESULTS： The best reversal effects on MDR of SGC7901/VCR cells were achieved with mdrlsi326, followed by mdrlsi2631, mdrlsi3071 and mdrlsi1513. Similarly, the best reversal effects on MDR of K562/A02 cells were also achieved with mdrlsi326, followed by mdrlsi2631, mdrlsi3071 and mdrlsi1513. CONCLUSION： The four siRNAs exerts similar reversal effects on multidrug resistance of both SGC7901/VCR and K562/A02 cells.

关 键 词：小干扰RNA MDR1基因 SGC7901/VCR细胞 K56/A02细胞 靶位点 

分 类 号：R73-3[医药卫生—肿瘤]