HCV NS3 N 末端部分氨基酸缺失对NS3/NS3与NS3/NS4A分子间相互作用的影响  

The influence of HCV N terminal deletion on the molecular interactions betweem NS3/NS3 and NS3/NS4A

作　　者：欧武[1,2] 杨翠红[1,2] 朱千政 邓小艺[1,2] 于曼 秦鄂德[1,2] 杨佩英 

机构地区：[1]军事医学科学院微生物与流行病研究所病毒室 [2]大连医科大学病理生理学教研室

出　　处：《中华微生物学和免疫学杂志》1998年第5期355-358,共4页Chinese Journal of Microbiology and Immunology

摘　　要：目的旨在弄清ＮＳ３参与分子间相互作用的确切区段，为研究针对ＮＳ３的抗ＨＣＶ寡肽小分子药物的设计提供依据。方法参照ＨＣＶ中国河北株序列设计ＮＳ３引物，将其Ｎ末端的前１５个和前３０个氨基酸分别缺失掉。然后用酵母双杂交系统检测ＮＳ３／ＮＳ３及ＮＳ３／ＮＳ４Ａ分子间相互作用强度在缺失前后的变化，从而判明ＮＳ３Ｎ末端氨基酸在分子间相互作用中的意义。核苷酸序列分析采用ＡｐｐｌｉｅｄＢｉｏｓｙｓｔｅｍ３７３Ａ型自动测序仪。结果ＮＳ３Ｎ末端氨基酸缺失前后，ＮＳ３／ＮＳ３分子间及ＮＳ３／ＮＳ４Ａ分子间相互作用的强度相差有显著性（Ｐ＜０．０１），但缺失１５个氨基酸和缺失３０个氨基酸对上述相互作用强度的影响差异无显著性（Ｐ＞０．０５）。结论ＮＳ３Ｎ末端的１～３０个氨基酸在ＮＳ３／ＮＳ３及ＮＳ３／ＮＳ４Ａ分子间相互作用中有一定意义，其Ｎ末端前１５个氨基酸（ＡＰＩＴＡＹＳＱＱＴＲＧＬＬＧ）对于分子间相互作用更为关键。本研究结果将为抗ＮＳ３丝氨酸蛋白酶活性的寡肽抑制物的研究打下基础。Objective The formation of NS3 serine protease crystal, NS3/NS4A complexity, and the results of two hybrid detection of NS3 and NS4A confirm the reality of interaction between NS3 and NS3, between NS3 and NS4A as well. But the precise domain of NS3, directly participated in the interactions, is not known now. This study is aimed at locating the domains directly participated in molecular interactions of NS3/NS3 and NS3/NS4A, and providing some valuable references to the research of anti HCV oligopeptide drug,which inhibits NS3 serine protease activity. Methods In designing NS3 primers, the sequences encoding the first fifteen and thirty amino acids of NS3 N terminal were deleted respectively. By comparing the strength between NS3/NS3 and NS3/NS4A molecular interaction, pre and post deletion the function of amino acids of NS3 N terminal in molecular interaction was analyzed. The applied biosystem 373A sequencer was used for DNA sequencing. Results The pre and post deletion interaction strength difference between NS3/NS3 and NS3/NS4A was significant( P <0.01), but the difference between 15 amino acids deletion and 30 amino acids deletion was insignificant( P >0.05). Conclusions Our study suggests that the first thirty amino acids are important in NS3/NS3 and NS3/NS4A interaction, and the first fifteen oligopeptides(APITAYSQQTRGLLG), located at N terminus of NS3, are more critical. This oligopeptides may be useful in designing the small molecule inhibitor of NS3 serine protease activity.

关 键 词：丙型肝炎病毒 氨基酸缺失 NS3 寡肽 药物设计 

分 类 号：R373.21[医药卫生—病原生物学] R965.1[医药卫生—基础医学]