产低温纤维素酶南极细菌的筛选、生长特性及酶学性质的初步研究  

Screening and growth of antarctic bacterium producing cold-active cellulose and its enzymatic characterization

作　　者：钱文佳[1] 阚光锋[1] 徐仲[1] 史翠娟[1] 雷振环[1] 

机构地区：[1]哈尔滨工业大学(威海)海洋学院,威海264209

出　　处：《食品科技》2010年第1期15-18,共4页Food Science and Technology

基　　金：山东省自然科学基金项目(Y2007D57);威海市科技计划项目(2007-82)

摘　　要：以南极海冰细菌作为实验材料,用刚果红染色和发酵培养法从64株细菌中筛选得到产纤维素酶菌株Pseudoalteromonassp.545。经生长条件优化,该菌以麦芽糖为碳源,蛋白胨为氮源,初始生长pH为8.0,10℃培养96h时产酶最高。对菌株粗酶液进行酶学性质初步研究,此酶在pH9.0、35℃下反应40min时酶活最高。另外,该菌株所产纤维素酶在0～60℃范围内均有活性,属于低温酶。Primarily screened by cellulose-congo red plate and then screened by shake flask fermentation, an effective cellulose-producing bacterium named Pseudoalteromonas sp. 545 was isolated from 64 antarctic bacteria strains. With maltose as carbon source, peptone used as nitrogen source, when 545 cultured at pH8.0 for 96 h under 10 ℃, it could produce the highest enzyme activity. Through the preliminary study of enzymatic characterization, the results showed the optimum pH of crude cellulose was 9.0, the optimum temperature 35 ℃ at 40 min. In addition, the cellulose kept its activity from 0 ℃ to 60 ℃, and belonged to low-temperature enzyme.

关 键 词：南极细菌 低温纤维素酶 筛选 生长特性 酶学性质 

分 类 号：TS201.3[轻工技术与工程—食品科学]