利用富马酸发酵废液培养L-天冬氨酸转化菌策略的研究  

Study on a new strategy for the cultivation of L-ASP-producing bacteria utilizing the wasted fumaric acid fermentation broth

作　　者：盛晓燕[1] 付永前[1] 李霜[1,2] 徐晴[1] 黄和[1,2] 

机构地区：[1]南京工业大学生物与制药工程学院,南京210009 [2]南京工业大学材料化学工程国家重点实验室,南京211816

出　　处：《食品科技》2010年第1期23-26,共4页Food Science and Technology

基　　金：国家自然科学基金项目(20706031);国家高科技研究发展计划“(863”计划)专题课题(2006AA02Z240);国家重点基础研究发展规划(973)基金项目(2007CB707805)

摘　　要：提出利用富马酸发酵废液培养天冬氨酸转化菌的新策略,着重考察发酵废液中不同碳氮源对L-天冬氨酸转化菌培养的影响。结果表明,当对富马酸发酵废液浓缩2.5～3.0倍时,此时富马酸与葡萄糖浓度达到14～16g/L,并添加浓度为2.82%的玉米干粉作为氮源时,菌体的酶活力可以达到153788U/g,转化率可以达到95%以上,从而进一步证实了利用酸发酵废液培养L-天冬氨酸转化菌策略的可行性,该策略的提出不仅为实现富马酸发酵废液的循环利用,同时也为探索更加经济有效的L-天冬氨酸的生产工艺提供了理论基础。This article proposes a new strategy for the cultivation of L-ASP-producing bacteria utilizing the wasted fumaric acid fermentation broth and mainly investigates the effect of different nitrogen sources of the wasted fumaric acid fermentation broth on the growth of L-ASP-producing bacteria. The results showed that the enzyme activity and the yield could be 153788 U/g and 95% respectively when the fumaric acid and glucose concentrations were between 14-16 g/L after concentrating the volume of the wasted fermentation broth to the range form 2.5-3 times with adding 2.82% of corn syrup powder concentration used as nitrogen sources. These proved the feasibility for cultivating L-ASP-producing strain using the wasted fumaric acid fermentation broth. Besides the recycle utilization of wasted fumaric acid fermentation broth, the strategy provide the strong basics for the production of L-ASP economically and efficently.

关 键 词：L-天冬氨酸 大肠杆菌 富马酸 发酵废液 化学需氧量 

分 类 号：TS201.3[轻工技术与工程—食品科学]