高效毛细管电泳法测定黄泥螺黏液中活性多糖的单糖组成  

Determination of monosaccharides in polysaccharides from the mucilage of Bullacta exarata by HPCE

作　　者：王翀[1] 宋伟[1] 夏照明[1] 许学书[1] 谢静莉[1] 

机构地区：[1]华东理工大学生物工程学院食品科学与工程系,上海200237

出　　处：《食品科技》2010年第1期274-276,281,共4页Food Science and Technology

摘　　要：目的:测定经提取纯化后黄泥螺黏液中3种活性多糖EBPA、EBPB1和EBPB2的单糖组成。方法:7种单糖标准品经α-萘胺衍生化以后,用不同浓度(55～85mmol/L)的硼砂作为电泳介质,探寻最佳电泳条件实现高效毛细管电泳分离,得到标准的a-萘胺衍生单糖的毛细管电泳区带电泳图谱。将提纯后的黄泥螺黏液中的3种活性多糖分别水解成单糖,用相同的方法在最佳电泳条件下经毛细管电泳分离,得到水解单糖的毛细管电泳图谱。结果:最佳工作条件:运行缓冲液为75mmol/L、pH10.4的硼砂水溶液,分离电压10kV,进样压力0.5Psi,进样时间10s,柱温25℃。通过与标准谱图对照分析,黄泥螺黏液中的活性多糖EBPA由鼠李糖、葡萄糖、甘露糖、阿拉伯糖和岩藻糖组成,EBPB1由鼠李糖、葡萄糖、甘露糖、阿拉伯糖、岩藻糖和半乳糖组成,EBPB2由葡萄糖、甘露糖、阿拉伯糖、岩藻糖和半乳糖组成。结论:高效毛细管电泳能有效分析多糖中单糖组分,灵敏度高,分离效果好。Objective： The method of HPCE was applied in the analysis of the monosaccharide components of three novel polysaccharides （EBPA, EBPB1, EBPB2） isolated from the mucilage of Bullacta exarata（BE）. Method： The monosaccharides were derived from α-naphthylamine and separated by HPCE in the borex buffer solution as carrier. The standard electropherogram of monosaccharides was analyzed. Polyssaccharides were hydrolyzed into monosaccharides and treated by the same method. The monosaccharides composition of polysaccharides were obtained. Result： the optimal operating conditions： 75 mmol/L sodium tetraborate buffer （pH 10.4）, 10 kV, 25℃, and 0.5 Psi pressure injection for 10 s. The monosaccharides compositions and molar ratios were determined. Conclusion： This method is simple, rapid, sensitive and can be used to determine the monosaccharides in polysaccharides of BE.

关 键 词：毛细管电泳 黄泥螺 多糖 单糖组分 

分 类 号：O657.8[理学—分析化学]