香菇EST资源的SSR信息分析  

Analysis of SSR Information in EST Resource of Lentinus edodes

作　　者：王艳芳[1] 赵彦宏[1] 王爱云[1] 王芹芹[1] 刘林德[1] 周瑞莲[1] 于晓飞[1] 

机构地区：[1]鲁东大学生命科学学院,山东烟台264025

出　　处：《食品科学》2010年第1期137-140,共4页Food Science

基　　金：国家自然科学基金项目(30800682;30860221);中央级公益性科研院所基本科研业务费专项基金项目(XJSYWFZX2008-22);鲁东大学科研基金项目(LY20073303)

摘　　要：对来源于NCBI公共数据库的12144条香菇(Lentinus edodes)EST序列进行简单重复序列SSR搜索。结果表明:在这些香菇EST序列中共有359个SSR位点,46种SSR的重复单元,其中2碱基重复243条(67.69%),3碱基重复114条(31.76%),5碱基重复1条(0.28%),6碱基重复1条(0.28%)。平均33.8条EST序列中有1个SSR。SSR的碱基总数达7727kb,约占整个EST序列碱基数的2.94%。本实验还根据搜索结果设计了100条香菇EST-SSR引物,并随机合成了20对引物。以4个野外采集的香菇DNA为模板,对引物进行了筛选与评价,有16对引物有较清晰且稳定的目标扩增产物,其中11对引物的产物存在遗传多态性。结果表明本实验设计开发的香菇的EST-SSR标记是可行且有效的。Simple sequence repeats（SSRs） were investigated in 12144 qualified ESTs downloaded from Nucleotide ESTs database of Lentinula edodes in NCBI.There were 359 SSRs in all the ESTs sequences of Lentinus edode,which were composed of 243 di-（67.69%）,114 tri-（31.76%）,1 penta-（0.28%） and 1 hexa-（0.28%） nucleotide SSRs.The estimated frequency of SSRs was approximately 1 SSR per 33.8 ESTs.Total length of SSRs was 7727 kb,accounting for about 2.94% of total ESTs sequences.In addition,we selected 100 ESTs sequences in Lentinus edodes and designed 100 primer pairs for EST-SSR markers,of which 20 were arbitrarily selected and synthesized.Selection and investigation of these primers were performed with 4 wild-type strains of Lentinula edodes.There were 16 primer pairs characterized by clear and stable target bands,of which 11 presented genetic diversity.Our findings indicated that these Lentinula edodes EST-SSRs markers developed in this study were useful and effective.

关 键 词：香菇 SSR EST 引物 

分 类 号：S646.1[农业科学—蔬菜学]