冬虫夏草与蛹虫草原生质体融合初探  

Protoplast Fusion between Cordyceps sinensis and Cordyceps militaris

作　　者：郭成金[1] 赵润[2] 朱文碧[3] 

机构地区：[1]天津师范大学化学与生命科学学院,天津市细胞遗传与分子调控重点实验室,天津300387 [2]农业部环境保护科研监测所,天津300191 [3]天津农学院教务处,天津300384

出　　处：《食品科学》2010年第1期165-171,共7页Food Science

基　　金：天津市科学技术委员会项目(06ZHCXNC05900)

摘　　要：采用正交设计和双灭活标记方法对冬虫夏草与蛹虫草进行原生质体融合研究,获得融合子。结果表明:最适融合条件为:40%PEG与0.08mol/LCa2+的混合溶液(pH7.0)助融,35℃融合20min,融合率为2.74×10-5;并用形态学、颉颃实验及分子生物学方法初步探讨了双亲和融合子三者之间的亲缘关系。Fusion between protoplasts from Cordyceps sinensis and Cordyceps militaris subjected to heat and ultraviolet inactivations,respectively,was studied.In order to maximize fusion rate,five crucial fusion conditions were optimized using orthogonal array design.A maximum fusion rate of 2.74 × 10-5 was achieved through 20 min fusion at 35 ℃ under the promotion of 40% PEG and 0.08 mmol/L CaCl2（pH 7）.Besides,the genetic relationships among the parent and fusant strains were quested by morphologic observation,rivalry experiments and RAPD-PCR identification.

关 键 词：冬虫夏草 蛹虫草 融合子 亲缘关系 

分 类 号：Q949.3[生物学—植物学]