微生物代谢组学的研究方法与进展  

Current Methods and Advances in Microbial Metabolomics

作　　者：王智文[1,2] 马向辉[1,2] 陈洵[1,2] 赵学明[1,2] 陈涛[1,2] 

机构地区：[1]天津大学化工学院生物工程系代谢工程和系统生物学实验室系 [2]统生物工程教育部重点实验室爱丁堡大学-天津大学系统生物学与合成生物学联合研究中心,天津300072

出　　处：《化学进展》2010年第1期163-172,共10页Progress in Chemistry

基　　金：国家重点基础研究发展计划(973)项目(No.2007CB707802);国家自然科学基金项目(No.20536040)资助

摘　　要：代谢组学、基因组学、转录组学和蛋白质组学是系统生物学研究的重要组成部分。近年来,在代谢组学领域,微生物代谢组学的研究受到人们的重视,成为研究的热点。本文综述了微生物代谢组学的研究方法,包括样品处理、分析平台、数据处理和生物学解释等,并讨论了微生物代谢组学在代谢工程方面的应用潜力,以及微生物代谢组学的研究前景和所面临的挑战。In the post-genomic era,metabolomics（or metabonomics）,genomics,transcriptomics and proteomics have become important tools in systems biology.In metabolome analysis field,microbial metabolomics has received much attention in recent years mainly because it supports and complements a wide range of microbial research areas from new drug discovery efforts to metabolic engineering.This review is mainly focused on the current methods and advances in microbial metabolomics,trying to direct the reader to the main approaches for microbial metabolome analysis,reviewing the developments of methodologies in microbial metabolomics.These developments range from sample preparation,analytical platform and data analysis to biological interpretation.Apart from the description of the developments,we will try to discuss the potential of microbial metabolomics in metabolic engineering field,research prospect and challenge of microbial metabolomics in the future.

关 键 词：微生物代谢组学 系统生物学 数据挖掘 代谢物 代谢工程 

分 类 号：Q591[生物学—生物化学] Q5-3