肺腺癌P53基因突变与临床病理特征关系的研究  

THE STUDY OF p53 GENE MUTATION ASSOCIATED WITH CLINICOPATHOLOGICAL CHARACTERISTICS IN LUNG ADENOCARCINOMA

作　　者：王德华[1] 白逸秋[2] 廖松林[3] 高子芬[3] 李宁[3] 张伟[3] 

机构地区：[1]湖北省郧阳医学院病理学教研室,十堰442000 [2]北京安贞医院病理科 [3]北京医科大学病理学系

出　　处：《中国癌症杂志》1998年第3期199-201,共3页China Oncology

摘　　要：目的探讨p53基因在肺腺癌中突变的频率、位置和在肺腺癌发生发展中的作用及与其临床病理特征的关系。方法聚合酶链式反应一单链构象多态性（PCR－SSCP）检测31例肺腺癌的P53基因第5～8外显子突变。结果14例（45％）出现P53基因5～8外显子突变，其中7、8外显子突变占73％。P53基因突变男性显著高于女性（P—0．003），在肿瘤≤3cm的病例p53基因突变率显著低于肿瘤＞3cm的病例（P=0．005）。p53基因突变与吸烟史、年龄、组织学类型、分化程度、淋巴结状况、国际病理TNM分期及瘤栓无显著性差异（P＞0．05）。结论肺腺癌中P53突变率为45％，主要分布在第7、8外显子，P53基因突变参与肺腺癌癌变的始动和腺癌的进展。To investigate the frequency, location, and the effects of p53 mutation in tumor progression. andto study possible correlation between p53 gene and the clinicopathological characteristics in lung adenocarcinoma.METHODS PCR-SSCP detected Exon5-8 of p53 gene mutation in 31 cases lung adenocarcinoma. RESULTS 14(45%) of 31 cases showed mutation in exon 5 to exon 8 of p53 gene. 73 % of the mutations occurred in exon 7 andexon 8. The frequency of p53 mutations was significantly higher in men than in women (P=0.003), and thefrequency of p53 mutations of tumor size more than 3 cm was significantly higher that of tumor size less than and equal to 3 cm (P=0. 005). There were no statistically significant differences in p53 status with respected to age,smoking, histological type, grade, lymphatic status, PTNM stage and tumor emboli (P>0. 05). CONCLUSIONThe frequency of p53 mutation is 45 % in lung adenocarcinoma. Most of the p53 mutations locate in exon 7 and 8.p53 gene mutations are involved either in the initiation of carcinogenesis and lung adenocarcinoma progression.

关 键 词：肺腺癌 P53基因 PCR 临床病理 基因突变 肺肿瘤 

分 类 号：R734.202[医药卫生—肿瘤]