人Glioblastoma/HHT多药耐药细胞系的建立及维拉帕米对耐药性的逆转作用  

Establishment of multidrug resistant cell line Glioblastoma/HHT and reversal of drug resistance by verapamil

作　　者：石淑文[1] 郭淑芬[1] 

机构地区：[1]浙江医科大学附属儿童医院

出　　处：《实用肿瘤杂志》1998年第5期272-275,共4页Journal of Practical Oncology

摘　　要：对人Ｇｌｉｏｂｌａｓｔｏｍａ细胞系采用反复短期暴露于高浓度的高三尖杉酯碱（ＨＨＴ）药液中的方法，建立了一株多药耐药（ＭＤＲ）细胞系，命名为Ｇｌｉｏｂｌａｓｔｏｍａ／ＨＨＴ。此细胞系高度表达Ｐ－糖蛋白（Ｐｇｐ），但无多药耐药相关蛋白（ＭＲＰ）的表达。５μｇ／ｍｌ维拉帕米（异搏定）能部分逆转Ｇｌｉｏｂｌａｓｔｏｍａ／ＨＨＴ细胞系的耐药性。２．５μｇ／ｍｌ～１０μｇ／ｍｌ异搏定不仅能增加Ｇｌｉｏｂｌａｓｔｏｍａ／ＨＨＴ细胞对柔红霉素（ＤＮＲ）的摄取量，也能减少ＤＮＲ的外排。We established a multidrug resistant (MDR) cell line Glioblastoma/HHT from Glioblastoma cell line by short and repeated exposures to high concentration of homoharringtonine(HHT).The MDR cell line overexpressed P glycoprotein(Pgp) but did not express MDR related protein(MRP).The resistance of Glioblastoma/HHT cell line could be partly reversed by 5 μg/ml verapamil.2.5 μg/ml～10 μg/ml verapamil was able to increase daunorubicin(DNR) accumulation and retention in Glioblastoma/HHT cells,and effect became increased along with the elevation in verapamil concentration.

关 键 词：胶质瘤 高三尖杉酯碱 GLIOBLASTOMA 维拉帕米 

分 类 号：R730.264[医药卫生—肿瘤] R979.1[医药卫生—临床医学]