Characterization of phenanthrene degradation by strain Polyporus sp. S133  

作　　者：Tony Hadibarata Sanro Tachibana 

机构地区：[1]Department of Applied Bioscience,Faculty of Agriculture,Ehime University [2]Laboratorium of Pulp and Paper,Department of Forest Technology,Mulawarman University Kampus Gunung Kelua

出　　处：《Journal of Environmental Sciences》2010年第1期142-149,共8页环境科学学报（英文版）

基　　金：supported by the Grant-in-aid for Scientific Research from the Ministry of Education,Culture,Sports,Science and Technology of Japan (No.20580178)

摘　　要：Polyporus sp. S133, a fungus collected from contaminated soil, was used to degrade phenanthrene, a polycyclic aromatic hydrocarbon, in a mineral salt broth liquid culture. A maximal degradation rate （92%） was obtained when Polyporus sp. S133 was cultured for 30 days with agitation at 120 r/min, as compared to 44% degradation in non-agitated cultures. Furthermore, the degradation was affected by the addition of surfactants. Tween 80 was the most suitable surfactant for the degradation of phenanthrene by Polyporus sp. S 133. The degradation rate increased as the amount of Tween 80 added increased. The rate in agitated cultures was about 2 times that in non-agitated cultures. The mechanism of degradation was determined through the identification of metabolites; 9,10-phenanthrenequinone, 2,2＇-diphenic acid, phthalic acid, and protocatechuic acid. Several enzymes （manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase） produced by Polyporus sp. S133 were detected during the incubation. The highest level of activity was shown by 1,2-dioxygenase （187.4 U/L） after 20 days of culture.Polyporus sp. S133, a fungus collected from contaminated soil, was used to degrade phenanthrene, a polycyclic aromatic hydrocarbon, in a mineral salt broth liquid culture. A maximal degradation rate （92%） was obtained when Polyporus sp. S133 was cultured for 30 days with agitation at 120 r/min, as compared to 44% degradation in non-agitated cultures. Furthermore, the degradation was affected by the addition of surfactants. Tween 80 was the most suitable surfactant for the degradation of phenanthrene by Polyporus sp. S 133. The degradation rate increased as the amount of Tween 80 added increased. The rate in agitated cultures was about 2 times that in non-agitated cultures. The mechanism of degradation was determined through the identification of metabolites; 9,10-phenanthrenequinone, 2,2＇-diphenic acid, phthalic acid, and protocatechuic acid. Several enzymes （manganese peroxidase, lignin peroxidase, laccase, 1,2-dioxygenase and 2,3-dioxygenase） produced by Polyporus sp. S133 were detected during the incubation. The highest level of activity was shown by 1,2-dioxygenase （187.4 U/L） after 20 days of culture.

关 键 词：PHENANTHRENE BIODEGRADATION METABOLITES Polyporus sp. S 133 

分 类 号：X172[环境科学与工程—环境科学]