小麦根际优势菌对畜禽用抗生素的敏感性研究  

Sensitivity to Veterinary Antibiotics of Dominant Bacteria in Wheat Rhizosphere

作　　者：杨清香[1] 张晶[1] 朱孔方[1] 俞宁 

机构地区：[1]河南师范大学生命科学学院,河南新乡453000 [2]河南省新乡市环境保护检测站,河南新乡453007

出　　处：《环境科学与技术》2010年第2期85-89,共5页Environmental Science & Technology

基　　金：国家自然科学基金项目资助(20677014)

摘　　要：文章通过培养的方法分析了小麦根际微生物的群落结构以及对常用畜禽用抗生素的敏感性,研究结果表明,小麦根际土壤微生物总数为5.251×108cfu/g,其中细菌占绝对优势,占总菌数的99.04%,放线菌占0.09%,真菌占0.06%。从中分离出10株优势细菌,经过分子生物学鉴定均与芽孢杆菌属(Bacillus)亲缘关系较近。这10株优势菌对土霉素和四环素的敏感性研究结果表明,大部分菌株对土霉素的敏感性高于四环素,不同的菌株对同一种抗生素的敏感性差异较大。10株菌对土霉素的敏感浓度在10～100μg/L之间,对四环素的敏感浓度在100～500μg/L之间。The impact of widely-used veterinary antibiotics on soil environment was studied. Results reveals that the rhizospheric microorganism of wheat is dominated by bacteria accounting for 99.04%, others being actinomyces and fungi. 10 strains of the dominant bacteria were isolated, which were classified and identified as the species of genus Bacillus. It was found that most of them were more sensitive to terramycin than to tetracycline, and there exist rather wide differences of sensitivity to a certain antibiotics among the isolated bacterial strains. As far as the concentrations of the studied antibiotics, bacteria＇s 100-500μg/L.

关 键 词：小麦根际 优势菌 抗生素敏感性 土霉素 四环素 

分 类 号：X172[环境科学与工程—环境科学]