5-氮-2′-脱氧胞苷对人脑星形细胞瘤细胞侵袭力的影响及机制探讨  

Effect and medchanism of 5-Aza-CdR on invasion ability of astrocytoma cells

作　　者：龚智泉[1] 孔令非[1,2] 赵跃武[2] 

机构地区：[1]郑州大学基础医学院,郑州450052 [2]河南省人民医院

出　　处：《山东医药》2010年第2期8-9,共2页Shandong Medical Journal

基　　金：河南省自然科学基金资助项目(0611041700)

摘　　要：目的观察特异性DNA甲基转移酶抑制剂5-氮-2′-脱氧胞苷(5-Aza-CdR)对人脑星形细胞瘤细胞侵袭力的影响,并探讨其机制。方法分别将终浓度为0、1×10-7、5×10-7、1×10-6mol/L 5-Aza-CdR加入星形细胞瘤细胞作用48 h,采用Boyden小室观察星形细胞瘤细胞的侵袭力,采用明胶酶谱分析法检测星形细胞瘤细胞基质金属蛋白酶9(MMP-9)的表达变化。结果随着5-Aza-CdR浓度的升高,星形细胞瘤细胞穿膜细胞数呈升高趋势,proMMP-9及MMP-9的表达呈增高趋势(P均<0.05)。结论5-Aza-CdR可增强人脑星形细胞瘤细胞侵袭力,可能与DNA低甲基化上调MMP-9表达有关。Objective To investigate the effect of 5-Aza-Z＇-deoxycytidine （5-Aza-CdR） on invasion of astrocytoma cells, and explore its mechanism. Methods Astrocytoma cells were treated with different concentrations of 5-Aza-CdR for 48 h. The invasion ability of astrocytoma ceils and the expression of MMP-9 were detected by Boyden chamber experiment and gelatin zymography. Results Along with the increase of the concentration of 5-Aza-CdR, the number of cells traversed through the membranes was on the rise; the expression of proMMP-9 and MMP-9 raised up gradually （P 〈 0.05 ）. Conclusion 5-Aza-CdR can enhance the invasion ability of astrocytoma cells, and it may be related to the upregulation of the expression of MMP-9 with DNA hypomethylation.

关 键 词：星形细胞瘤 基质金属蛋白酶 甲基转移酶抑制剂 5-氮-2’-脱氧胞苷 

分 类 号：R739.4[医药卫生—肿瘤]