神经节苷脂GM3、GD3对小鼠巨噬细胞介导的细胞毒作用的影响  

EFFECT OF GANGLIOSIDE GM3 AND GD3 ON THE CYTOTOXICITY OF MOUSE MACROPHAGE

作　　者：吴欣[1] 刘银坤[1] 

机构地区：[1]上海医科大学肝癌研究所

出　　处：《肿瘤》1998年第5期334-337,共4页Tumor

基　　金：国家自然科学基金

摘　　要：目的观察外源性神经节苷脂ＧＭ３、ＧＤ３对人肝癌ＳＭＭＣ┐７７２１细胞的杀伤活力的影响。方法应用３Ｈ┐ＴｄＲ掺入法测定ＧＭ３、ＧＤ３对巨噬细胞的细胞毒作用的影响。结果ＧＭ３、ＧＤ３对巨噬细胞短时间（４５分钟）作用后，两者都对细胞毒产生双相调节，但两者调节作用相反。ＧＭ３、ＧＤ３对巨噬细胞长时间（２４小时）作用后，两者均抑制细胞毒作用，ＧＤ３的抑制作用更强。结论ＧＭ３、ＧＤ３作用于巨噬细胞后对其杀伤ＳＭＭＣ┐７７２１细胞的细胞毒作用产生不同程度的抑制。Objective:The cytotoxicity mediated by macrophage treated with ganglioside GM3 and GD3 on SMMC7721 cell line were detected in this study. Methods:The effect of GM3 and GD3 on cytotoxicity of macrophage was observed by the ~3HTdR incorporation.Results:Both GM3 and GD3 showed bifunctional effects on cytotoxicity after incubating with different concentration of GM3 or GD3 for a short time(45 min). The data indicated that GM3 could reduce the cytotoxicity at the lower concentration(0.1～1 mg/L) and increase it at higher concentration(10 mg/L);GD3 expressed its function in a reverse way. Treating macrophage for a long time(24 hours),it was found that both GM3 and GD3 inhibited the cytotoxicity. However,the effect of GD3 was more efficient than GM3. Conclusion:The results suggested that GM3 and GD3 may play a role in host immune surveillance and immune destruction mediated by macrophage.

关 键 词：神经节苷脂 GM3 GD3 巨噬细胞 细胞毒作用 肝癌 

分 类 号：R730.3[医药卫生—肿瘤] R735.703[医药卫生—临床医学]