不同提取剂对红花桑寄生叶抗氧化活性的影响  

Effects of Different Extraction Solvents on the Antioxidant Activities of Leaves Extracts of Scurrula parasitica

作　　者：陈炳华[1] 赖京菁[1] 郑琴[1] 李均[1] 肖义军[1] 

机构地区：[1]福建师范大学生命科学学院,福建福州350108

出　　处：《福建师范大学学报（自然科学版）》2010年第1期86-90,共5页Journal of Fujian Normal University：Natural Science Edition

基　　金：福建省教育厅资助项目(JA05201);福建省大学生创新性实验计划项目(2007-011);福建师范大学国家级生物学实验教学示范中心本科生创新性研究计划项目(2009ls007)

摘　　要：测定了红花桑寄生(寄主夹竹桃)叶的水、体积分数80%甲醇和体积分数80%丙酮3种提取物抗氧化活性.结果表明:3种溶剂提取物均具有自由基清除能力,在3种溶剂提取物中,体积分数80%丙酮提取物清除DPPH自由基能力最强(ρEC50=0.306mg/mL),抗氧化能力最强,FRAP和TEAC值分别为0.81,1.62.3种提取物抗氧化活性从高至低依次为丙酮提取物、甲醇提取物、水提取物.此外,体积分数80%丙酮提取物中总酚(22.89%)和总黄酮(11.61%)也高于其他2种.可见,提取剂的性质对红花桑寄生叶提取物抗氧化活性的影响显著.Water,80% methanol and 80% acetone extracts of leaves of Scurrula parasitica （parasitic on Nerium indicum）,were examined for their antioxidant activities by various assay.All leaves extracts were capable of free radicals scavenging activities. Among three solvent extracts of S.parasitica leaves,80% acetone extract showed the highest DPPH radical scavenging capacity with the value of ρEC50 0.306 mg/mL,and had the strongest antioxidant activity at 0.81 and 1.62 for FRAP and TEAC assays. The antioxidant activities of different extracts were in this order. 80% acetone extract, methanol extract, water extract. In addition, 80% acetone extract contained more phenolics （22.89%） and flavonoids （11.61%） than the other two extracts. Therefore, the properties of the extracting solvents significantly affected the antioxidant activity of leaf extract from S. barasitica.

关 键 词：红花桑寄生 叶提取物 抗氧化活性 提取剂 

分 类 号：Q946.91[生物学—植物学] S792.95[农业科学—林木遗传育种]