白藜芦醇对小鼠3T3-L1细胞增殖与分化的影响及其机制  

Effects of resveratrol on proliferation and differentiation of murine 3T3-L1 preadipocytes and the underlying mechanisms

作　　者：陈思凡[1] 肖新才[2] 孙延双[1] 郑琳[1] 冯翔[1] 

机构地区：[1]中山大学公共卫生学院 [2]广州市疾病预防控制中心,广东广州510089

出　　处：《中国药理学通报》2010年第1期108-111,共4页Chinese Pharmacological Bulletin

基　　金：广东省医学科研基金资助项目(No2008YK0653)

摘　　要：目的探讨白藜芦醇(resveratrol,Res)对小鼠3T3-L1细胞增殖、分化的影响及其机制。方法培养3T3-L1前脂肪细胞,添加不同剂量的白藜芦醇,用WST-1法检测细胞的增殖;用油红O染色后以染色比色法分析脂肪细胞的分化程度;采用RealtimePCR技术检测各组脂联素和瘦素的mRNA表达;采用Westernblot技术检测相关位点,包括沉默信息调节因子1(silent information regulator 1,Sirt1)、过氧化物酶增殖物激活受体γ(peroxisome proliferator activated re-ceptor γ,PPARγ)、CCAAT/增强子结合蛋白α(CCTT Aenhan-cerbinding proteinα,C/EBPα)的蛋白质表达。结果Res明显抑制3T3-L1前脂肪细胞增殖,且呈一定的时间-剂量依赖关系;与对照组比较,Res组脂联素和瘦素的mRNA表达水平下降(P<0.01),Res抑制3T3-L1前脂肪细胞向成熟脂肪细胞分化;添加Res导致脂肪细胞中Sirt1蛋白表达水平上升,PPARγ和C/EBPα蛋白表达水平下降。结论白藜芦醇能抑制3T3-L1前脂肪细胞增殖和分化,其机制可能是通过增加Sirt1表达,抑制脂肪细胞分化相关蛋白PPARγ、C/EBPα的表达。Aim To explore the effect and mechanism of resveratrol （Res） on proliferation and differentiation of murine 3T3-L1 preadipocytes. Methods 3T3-L1 preadipocytes were cultured and treated with resveratrol in different dosages. Cell proliferation was analyzed by WST-1 method. Oil red O staining method and spectro-photography were applied to analyze the degree of differentiation. Real-time PCR was applied to detect the mRNA expression of adiponectin and leptin. Western blot was applied to detect the expression of silent information regulator 1 （Sirt1）,peroxisome proliferator activated receptor γ （PPARγ） and CCTTA enhancer binding proteinα （C/EBPα）. Results Res inhibited proliferation of murine 3T3-L1 preadipocytes in a time-dose dependent manner. The expression levels of adiponectin and leptin mRNA were decreased,and Res also inhibited 3T3-L1 preadipocytes to differentiate into mature adipocytes. Res increased the expression levels of Sirt1 and decreased the expression levels of PPARγ and C/EBPα. Conclusions Resveratrol can inhibit the proliferation and differentiation of 3T3-L1 preadipocytes. The underlying mechanisms may include enhancing expression of Sirt1 and inhibiting expression of PPARγ,C/EBPα which are related to cell differentiation.

关 键 词：白藜芦醇 脂肪细胞 脂联素 瘦素 沉默信息调节因子1 过氧化物酶增殖物激活受体Γ CCAAT/增强子结合蛋白α 

分 类 号：R-332[医药卫生] R282.71