无指盘臭蛙皮肤抗菌肽对5种常见病原菌的体外抑菌活性研究  

In vitro Activities of Odorrana grahami Antimicrobial Peptides from Skin Against 5 Pathogenic Bacteria

作　　者：刘炯宇[1] 张砺[2] 江建平[1] 王晓蕾[2] 谢锋[1] 郑中华[1] 

机构地区：[1]中国科学院成都生物研究所,四川成都610041 [2]成都市儿童医院,四川成都610017

出　　处：《中药材》2009年第12期1807-1810,共4页Journal of Chinese Medicinal Materials

基　　金：四川省科技厅攻关项目(04SG011-024;2006Z08-006;2008JY0001);中国科学院知识创新工程青年人才领域前沿项目(CIB-2007-LYQY-01);国家自然科学基金(30800100)

摘　　要：目的:测试4种天然无指盘臭蛙皮肤抗菌肽Odorgrin A、Odorgrin B、Odorgrin C和Odorgrin D纯品对5种临床常见病原菌(其中野生菌2株,耐药菌7株)的抑菌活性。方法:采用微量液体稀释法进行体外抑菌试验。结果:4种天然无指盘臭蛙皮肤抗菌肽对所测试的耐甲氧西林凝固酶阴性葡萄球菌(85460),野生型金黄色葡萄球菌(24157),耐青霉素肺炎链球菌(84688和91452),产Ⅰ型β内酰胺酶阴沟肠杆菌(85439和93543),野生型大肠埃希菌(21512),产超广谱β内酰胺酶大肠埃希菌(84492),耐酶抑制剂的β内酰胺酶大肠埃希菌(85580)大都具有较强的体外抑制作用。结论:4种天然无指盘臭蛙皮肤抗菌肽均具有广谱抗菌活性,对耐药菌株也有较好的体外抑菌能力。4种天然无指盘臭蛙皮肤抗菌肽有良好的应用前景,具有开发成为抗菌药物以及消毒剂和防腐剂的潜力。Objective：To evaluate the efficiency of 4 Odorrana grahami antimicrobial peptides from skin against 5 pathogenic bac- teria,include 2 wild strains and 7 resistant strains.Methods：Broth microdilution antimicrobial susceptibility test was used for bacteria that growed aerobically.Results：The 4 Odorrana grahami antimicrobial peptides were basically in vitro efficient agents for inhibition against to methicillin-resistant coagulase negative Staphylococcus（MBSCN,85460）,wild Staphylococcus aureus（24157）,penicillin-resistant Streptococcus pneumoniae（PRSP,84688 and 91452）,class Iβ-lactamase Enterobacter cloacae（AmpC,85439 and 93543）,wild Escherichia coli（84492）,extended-spectrumβ-lactamases Escherichia coli（ESBL,84492）,inhabitor-resistant TEMβ-lactamase Escherichia coli（IRT,85580）.Conclusion：The 4 Odorrana grahami antimicrobial peptides from skin have broad spectrum antimicrobial activity; especially have in vitro activity to resistant strains.So it is hopeful to be developed as the antimicrobial agent as well as the disinfectant and the antiseptic.

关 键 词：无指盘臭蛙 皮肤抗菌肽 耐药菌株 抑菌 

分 类 号：R282.05[医药卫生—中药学]