survivin反义寡核苷酸诱导SGC7901细胞凋亡及增强紫杉醇敏感性  

Survivin antisense oligonucleotide induces apoptosis of SGC7901 cells and enhances sensitivity to taxol

作　　者：何金兰[1] 孔庆兖[1] 

机构地区：[1]徐州医学院病理教研室,徐州221002

出　　处：《临床与实验病理学杂志》2009年第6期619-623,共5页Chinese Journal of Clinical and Experimental Pathology

基　　金：江苏省教育厅自然科学基金(04KJB310143)

摘　　要：目的研究转染生存素(survivin)反义寡核苷酸(Asodn)抗肿瘤作用以及是否增强紫杉醇敏感性。方法实验分为:空白对照组(Control组)、脂质体组(Lip组)、正义寡核苷酸组(Sodn组)及反义寡核苷酸组(Asodn组)。人工合成Asodn,经脂质体转染SGC7901细胞,MTT检测细胞增值抑制率,Western blot检测survivin蛋白表达,Hoechst33258染色荧光显微镜下观察细胞凋亡形态学改变及流式细胞仪检测细胞凋亡率。结果①荧光显微镜下Control组、Lip组及Sodn组细胞核呈均匀蓝色、圆形或椭圆形,而Asodn组细胞核染色增强,核质浓缩,核碎裂;②Asodn组细胞增殖抑制率增高,并呈时间-剂量依赖性;survivin蛋白表达减低;凋亡率增高。与Control组、Lip组及Sodn组相比,差异均有统计学意义(P<0.05);③Asodn联合紫杉醇组其抑制率(78.1±0.8)%明显高于单纯Asodn组(54.9±1.6)%和紫杉醇组(56.7±0.7)%(P<0.05)。结论转染Asodn能明显抑制SGC7901细胞增殖、诱导凋亡并增强紫杉醇抗肿瘤作用。Purpose To investigate the anti-tumor effect of survivin antisense oligonucleotide（Asodn）,and whether it can strengthen the sensitivity of chemical therapy with taxol.Methods The experiment was devided into four groups：control group,liposome group, sense oligonucleotide group,and antisense oligonucleotide group.Survivin antisense oligonucleotide was synthesized,and transfected into gastric cancer cells with liposome.The inhibitory rate of proliferation was tested with MTT,and the expression of survivin protein with Western blot;the morphological changes of apoptosis through Hoechst staining were observed,and the apoptosis rate with flow cytometer. Results As observed through Hoechst staining,the cancer cells had normal blue nucleus in the control group,Lip group,and Sodn group,while in Asodn transfection group the nucleus became condensed,with karyorrhexis.The cell inhibitoty rate in Asodn group increased,presenting in a time-dosage dependence manner;survivin protein expression reduced and apoptotic rate increased; there were differences in statistical significance（P〈0.05）,as compared with the control group,Lip group and Sodn group.The proliferation -inhibitory rate in Asodn plus taxol group[（78.1±0.8）%]was obviously higher than that in Asodn group[（54.9±1.6）%]and taxol group[（56.7%±0.7）%]（P〈0.05）.Conclusion Survivin antisense oligonucleotide can inhibit the proliferation of gastric cancer cells,induce apoptosis,and strengthen the effect of taxol on the inhibition of tumor growth.

关 键 词：生存素 SGC7901细胞 反义寡核苷酸 紫杉醇 细胞凋亡 

分 类 号：R735.2[医药卫生—肿瘤]