超抗原SEA对C57BL/6荷瘤小鼠S180肉瘤的抑制检测及肿瘤坏死因子的分析  

Inhibitory Effect of SEA on S180 Growing in C57BL/6 Mouse and Analysis of the Tumor Necrosis Factor

作　　者：陈自辉[1] 孙嘉琳[1] 张云[1] 王雪[1] 冯艳敏[1] 张洪娜[1] 

机构地区：[1]天津科技大学生物工程学院,天津300457

出　　处：《现代食品科技》2010年第1期43-45,共3页Modern Food Science and Technology

基　　金：天津市科委资助的应用基础研究重点项目(06YFJZJC02600)

摘　　要：通过在C57BL/6小鼠腋下注射S180肉瘤细胞,隔日注射生理盐水(对照组)和葡萄球菌肠毒素A(staphylococcal enterotoxin A,SEA)(实验组),九日后解剖小鼠测量抑瘤率和脾指数,并通过ELISA和免疫组化方法检测TNF-α。解剖后测量发现实验组小鼠瘤重较对照组轻,分别为(0.52±0.10)g和(1.02±0.11)g(P<0.05);两组的脾指数分别为15.9和4.8;同时发现实验组脾脏和血清中TNF-α的含量均高于对照组。因此认为超抗原SEA可以有效提高小鼠的免疫能力,从而更高效的杀伤癌细胞。This experiment studies the anti-tumor effect of the staphylococcal enterotoxin A on S180 solid tumor in C57BL/6 mouse modle and detect the anti-tumor rote and the spleen index. And the TNF-α content in the spleen and blood serum was also studied through ELISA and immunohistochemistry. S 180 cells was injected to the oxter of the C57BL/6 to build up the solid tumor mouse model, then physiologic saline （conlrol） and SEA（test） were inttapedtoneally injected to these mouse every two days. Nine days later, the mousse were dissected to detect anti-tumor rate, spleen index and TNF-α .It was found that the weights of solid tumor in the test group （0.52±0.10 g ） were lighter than the control group （ 1.02±0.11 g ） （P〈0.05）. The spleen indexes of the two groups were 15.9 and 4.8, respectively. Results also showed that TNF-α content in the test group was more than that in the conlrol group. It was concluded that SEA can effectively improve the immunity of the mouses, thus killing the tumor cells in mouse model group.

关 键 词：金黄色葡萄球菌肠毒素A(SEA) ELISA 免疫组织化学 抑瘤率 脾指数 TNF-α 

分 类 号：R73-3[医药卫生—肿瘤]