响应面法优化獐牙菜苦苷生物转化条件  

Biotransformation optimization of swertiamarin by response surface methodology

作　　者：常军[1] 胡娜[1] 张铁军[2] 刘昌孝[2] 田成旺[2] 

机构地区：[1]江西科技师范学院南昌市天然产物与活性物质重点实验室,南昌330013 [2]天津药物研究院,天津300193

出　　处：《生物加工过程》2010年第1期28-33,共6页Chinese Journal of Bioprocess Engineering

基　　金：天津市应用基础重点资助项目(08jczDJc24700);江西科技师范学院博士基金资助项目

摘　　要：采用响应面法对獐牙菜苦苷的生物转化条件进行优化,首先应用Plackett-Burman方法筛选出对转化具有显著性影响的因素,再用中心响应面法的Response Optimizer工具对最优转化条件进行了分析和预测,最后对预测结果进行了验证。结果表明,獐牙菜新碱的最优化转化条件(mg/mL)为MgSO4.7H2O 5.14、MnSO4.4H2O 3.42、葡萄糖9.95、蛋白胨9.24、pH5.80。在此条件下獐牙菜新碱的最大转化产率为17.64%;红百金花内酯的最优转化条件(mg/mL)为MgSO4.7H2O 4.09、MnSO4.4H2O6.07、葡萄糖7.37、酵母膏7.37,pH5.73。在此条件下红百金花内酯的最大转化产率为8.81%。Biotransformation conditions of swertiamarin were optimized by response surface methodology. The Plackett-Burman method was firstly used to screen significant factors on the biotransformation. Cen- tral response surface method was used to analyze the biotransformation and the response optimizer toot was used to estimate the optimal biotransformation conditions of swertiamarin to alkaloid and erythrocentaurin, respectively. Finally, the optimal conditions were verified by fermentation test. The optimal condition for the alkaloid formation was （in mg,/mL） ： MgSO4 .7H20 5.14, MnSO4 ·4H20 3.42, glucose 9.95, pep- tone 9. 24, and pH 5.80, the maximum recovery was 17.64%. The optimal condition for erythroeentaurin was （in mg/mL） ： MgSO4 ·7H2O 4.09, MnSO4·4H2O 6. 07, glucose 7.37, peptone 7.37, and pH 5.73, the maximum recovery was 8.81%.

关 键 词：獐牙菜苦苷 生物转化 獐牙菜新碱 红百金花内酯 

分 类 号：TQ920.1[轻工技术与工程—发酵工程]