微量生色底物法测定肝素钠抗Ⅹa因子和抗Ⅱa因子活性  

Determination of the activity of anti-FⅩa and anti-FⅡa of heparin by micro-chromogenic substrate methods

作　　者：李京[1] 范慧红[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《中国生化药物杂志》2010年第1期45-47,共3页Chinese Journal of Biochemical Pharmaceutics

摘　　要：目的英国药典中收载了半微量生色底物法测定低分子肝素抗Ⅹa因子和抗Ⅱa因子活性,在此基础上,我们考虑是否可以将实验方法进行改进,用微量生色底物法测定肝素类药物活性。方法调整样品与试剂浓度,降低总反应体积,在96孔酶标板中进行反应,用酶标仪测定样品吸光度。结果对6个肝素样品进行抗Ⅹa因子和抗Ⅱa因子活性测定,实验结果误差小,重复性良好。结论微量生色底物法可以用于肝素类药物抗Ⅹa因子和抗Ⅱa因子活性测定,该法提高了实验效率,降低了检验成本,可以考虑用本法取代传统的凝血法测定肝素效价。Purpose Semi-macro-chromogenic substrate methods have been adopted by British Pharmacopoeia to determine the anti-FⅩa activity and anti-F n a activity of low molecular weight heparin. Based on the above methods,the micro-chromogenic substrate methods were established to determine the anti-FⅩa and anti-FⅡa activity of heparins. Methods After the adjustment of the concentration of reagents and the whole volume of the test,96-well microplates and the microplate spectrophotometer have been used to determine the absorbance. Results Six samples of heparin＇s potencies of anti-F X a and anti-FⅡa have been determined. The test results show that the errors of the tests are small and the repeatability is good. Conclusion Micro-chro- mogenic method can be used to determine the activity of anti-FⅩa and anti-FⅡa of heparins. This method im- proved the efficiency of the test and decreased the cost of the test. Now the chromogenie substrate methods are being considered as the pharmacopoeia methods to substitute the anticoagulant methods.

关 键 词：肝素 抗Ⅹa因子活性 抗Ⅱa因子活性 生色底物法 

分 类 号：R927.2[医药卫生—药学]