MLF植物乳杆菌R23培养基优化  

Optimization of culture medium for Lactobacillus plantarum R23 for malotactic fermentation

作　　者：梁璋成[1,2] 何志刚[1] 任香芸[1] 李维新[1] 林晓姿[1] 陈江华 

机构地区：[1]福建省农业科学院农业工程技术研究所,福建福州350003 [2]福建省农林大学食品科学学院,福建福州350002 [3]福建省福清市出入境检验检疫局,福建福清350300

出　　处：《福建农业学报》2009年第6期570-574,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2008J0056);福建省科技计划项目(2008N0021);福建省公益类科研院所基本科研专项(2009R10033-3);福建省财政专项--福建省农业科学院科技创新团队建设基金(STIF-Y05)

摘　　要：根据乳酸菌的营养需求,采用均匀设计优选具有苹果酸乳酸发酵能力植物乳杆菌R23生长的培养基组分,并对培养基进行正交优化,筛选出无Mn2+及含Mn2+培养基LH16、LH18组合。采用LH16、LH18培养基培养植物乳杆菌R23,在25℃恒温培养24 h,菌量分别达到3.40×109cfu.mL-1和6.67×109cfu.mL-1。Based on the nutritional requirements of lactic acid bacteria, the uniform design was used to optimize the formulation of culture medium suitable for the growth of the lactobacillus plantarum for the malolactic fermentation. Orthogonal optimization was further applied for the selection of the LH16 （non-Mn^2＋ ） and LH18 （with Mn^2＋ ） medium. By incubating the lactohacillus plantarum in the LH16 and LH18 medium at 25℃ for 24h, the cell densities were 3.40 × 10^9 and 6.67 × 10^9 cfu per mL, respectively. They were twice as high as the MRS medium could produce.

关 键 词：苹果酸乳酸发酵 植物乳杆菌 培养基 

分 类 号：S201[农业科学—农业工程]