短梗霉多糖菌的复合诱变筛选及培养基优化  

Combining Mutation of Pullulan Producing Srains and Optimization on Fermentation Medium

作　　者：朱永强 方尚玲[1] 覃敬羽[1] 王清[1] 李世杰[1] 

机构地区：[1]发酵工程省部共建教育部重点实验室,武汉湖北工业大学生物工程学院,430068

出　　处：《食品与发酵科技》2010年第1期47-50,共4页Food and Fermentation Science & Technology

摘　　要：一株经60Co照射后在平板上不产色素的出芽短梗霉作为出发菌株,经紫外诱变,亚硝酸诱变处理,摇瓶发酵筛选得一株短梗霉多糖产量较高的菌株3#-01。通过正交试验确定了3#-01菌株的优化摇瓶培养基,摇瓶发酵液颜色浅白,在优化培养基上短梗霉多糖产量可达35.77g/L,对葡萄糖转化率达63.8%。An Aureobasidium pullulans strain of color free colony on plate culture has been previously treated by 60Co γ radial as original strain. It was treated by UV radial and with HNO2 respectively. A high pullulan production mutant 3^#-01 was screen out by shake-flask test. An optimized culture medium was confirmed for the mutant by further shake fermentation through orthogonal experiment. The pullulan production was 35.77g/L, and the yield to glucose reached 63.8% from the optimized culture.

关 键 词：出芽短梗霉 诱变 短梗霉多糖 培养基优化 

分 类 号：TS261.1[轻工技术与工程—发酵工程]