番茄红素β环化酶基因RNAi植物表达载体的构建及遗传转化  

The Construction of Plant Vector Construction of Lycopene Cyclase-β Gene RNAi and Transformation

作　　者：马超[1] 马兵钢[1] 郝青南[1] 何娟[1] 王蕾[1] 

机构地区：[1]石河子大学农学院园艺系,石河子832003

出　　处：《石河子大学学报（自然科学版）》2010年第1期28-32,共5页Journal of Shihezi University(Natural Science)

基　　金：国家自然科学基金项目(30460081);新疆兵团博士基金项目(ZD2007JC06)

摘　　要：番茄红素是类胡萝卜素合成途径中重要的中间产物,本研究探讨了在番茄中通过RNAi手段提高番茄红素含量的依据,由Genbank中番茄红素β环化酶(Lyc-β)序列设计2对引物,通过高保真PCR扩增出2段Lyc-β基因的高度保守片段。根据RNAi的原理将该基因片段构建成为具有RNA干扰作用的植物表达载体,将该干扰载体通过农杆菌介导转化的叶盘法转入烟草中。经过PCR鉴定证明,干扰载体已经转入烟草中。Lycopene is an important intermediate product of synthetic process of carotenoid. Some technique for increasing the content of lycopene in tomatoes by means of RNA interference is discussed. The two pairs of primers are designed according to the Lycopene cyclase-β gene sequence in GenBank, then get two segments by PCR from the tomato cDNA which is reversely transcribed from tomato total RNA. The two segments are used to construct the RNA interference expression vector. The vector is transformed into the tobaccos through the Agrobacterium tumefacien.

关 键 词：类胡萝卜素 番茄红素 β环化酶 RNAI 

分 类 号：S641.2[农业科学—蔬菜学] Q785[农业科学—园艺学]