抗菌肽与碱性成纤维细胞生长因子基因的融合及其在酵母中的表达  

Gene Fusion of Cecropin D with bFGF and Expression in Yeast

作　　者：吴映雅[1,2] 刘飞鹏[1,2] 周天鸿 朱嘉明[1,2] 李月琴 

机构地区：[1]广州中医药大学生化教研室 [2]暨南大学生物工程系

出　　处：《生物工程进展》1998年第5期11-15,共5页Progress in Biotechnology

基　　金：广东省自然科学基金

摘　　要：从重组质粒ｒＢＳ上切下柞蚕抗菌肽Ｄ基因片段，切去终止密码后连接到重组穿梭质粒ｐＶＴ－ＧＦ上碱性成纤维细胞生长因子ｃＤＮＡ的５′端，使密码框正确排列，构建成融合基因重组质粒ｐＶＴ－ＣＤＧＦ，转化到酵母中进行表达。转化子酵母蛋白粗提物用Ｅ．ｃｏｌｉＫ１２Ｄ３１作指示菌进行抑菌圈测试，初步检出具有抑菌活性，用ＥＬＩＳＡ检测证明其具有碱性成纤维细胞生长因子的抗原性。Cecropin D gene was isolated from plasmid rBS. Then its termination codon was removed and it was genetically fused to the 5′ end of b.bFGF cDNA in the E.coli Yeast shuttle vector pVT GF. The recombinant plasmids pVT CDGF were transformed into yeasts MGG 3 by electroporation. The expressed raw product exhibited antibacterial activity and the antigenicity for bFGF.

关 键 词：抗菌肽 穿梭质粒 融合基因 FGF 表达 酵母 

分 类 号：Q78[生物学—分子生物学]