溶氧对假单胞菌TS1138生产L-半胱氨酸合成酶系的影响  

Effect of Dissolved Oxygen on Production of L-cysteine Synthetase by Pseudomonas sp. TS1138

作　　者：怀丽华[1] 陈宁[2] 

机构地区：[1]天津商业大学商学院,天津300134 [2]天津科技大学生物工程学院,天津300457

出　　处：《食品科学》2010年第3期182-184,共3页Food Science

基　　金：天津市科委应用基础研究重点基金项目(05YFJZJC00901)

摘　　要：假单胞菌(Pseudomonas sp.)TS1138在含有DL-2-氨基-Δ2-噻唑啉-4-羧酸(DL-2-amino-Δ2-thiazoline-4-car-boxylic acid,DL-ATC)的环境中能够转化DL-ATC生成L-半胱氨酸的酶系。通过固定不同溶氧浓度,探索溶氧对假单胞菌TS1138发酵产酶的影响。摇瓶实验结果表明:溶氧浓度过低,菌体生长缓慢,细胞产酶能力较低;溶氧浓度过高,菌体生长速度快,但不利于细胞产酶。7L罐发酵实验结果表明:在产酶中后期,应根据溶氧浓度变化来调节搅拌转速或空气流量,使溶氧浓度控制在30%以上。Pseudomonas sp.TS1138 has potential to produce L-cysteine synthetase through asymmetrical hydrolysis of DL-2-amino-Δ2-thiazoline-4-carboxylic acid（DL-ATC）.The effect of dissolved oxygen level on the production of L-cysteine synthetase was investigated in shake flasks or 7 L bioreactor.Results indicated that the cell growth and the production of L-cysteine synthetase were inhibited at low dissolved oxygen level.Although cell growth was improved at the high dissolved oxygen level,the inhibition against production of L-cysteine synthetase was still observed in shake flasks.In 7 L bioreactor,dissolved oxygen concentration controlled at more than 30% was helpful for improving the cell growth and the production of L-cysteine synthetase through regulating agitation rate and air flow rate during the middle and late stage.

关 键 词：L-半胱氨酸 溶氧 假单胞菌 

分 类 号：TQ464.7[化学工程—制药化工] TQ920.1[轻工技术与工程—发酵工程]