产氢反应器内两种发酵类型细菌的种群结构与发酵特征  

Microbial community group characteristics for two types of fermentative hydrogen-production in a hydrogen producing reactor

作　　者：宋佳秀[1] 任南琪[2] 张永明[1] 陈瑛[2] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234 [2]哈尔滨工业大学市政环境工程学院,哈尔滨150090

出　　处：《环境科学学报》2010年第2期321-326,共6页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金(No.50808049);上海师范大学一般科研项目基金(No.DKL842);上海师范大学重点学科资助项目基金(No.DZL711)~~

摘　　要：通过改变CSTR系统内pH值,启动发酵类型由乙醇型向丁酸型转化,研究了转化前后系统内产氢动态和细菌群落变迁.结果表明,在有机负荷不变的情况下,启动发酵类型转化15d后,系统内种群由乙醇型转化为丁酸型,消耗碱度量由350mg.L-1增至1720mg.L-1,平均比产氢速率由21.2mol.kg-.1d-1降低至11.1mol.kg-.1d-1.荧光原位杂交技术(FISH)对反应系统内3类微生物群的监测结果表明,未启动转化时,肠杆菌、梭菌Ⅰ、Ⅱ和梭菌Ⅺ的相对丰度分别为22%、48%和30%,转化结束后,3类细菌的相对丰度为19%、24%和55%.The hydrogen-producing system and the bacterial community in a continuous stirred-tank reactor （CSTR） were studied before and after converting the process from ethanol-type fermentation to butyric acid-type by changing the pH.The results showed that the type of fermentation system transformed after 15 d from ethanol into acid-based type with organic loading unchanged.Alkalinity consumption increased from 350 mg·L^-1 to 1720 mg·L^-1 and the average hydrogen production rate changed from 21.2 mol·kg^-1·d^-1 to 11.1 mol·kg^-1·d^-1.Three types of microorganisms were detected in the reactor by fluorescence in situ hybridization （FISH）.Before the process,the relative abundances of Enterobacter,Clostridium cluster I,II and Clostridium cluster XI were 22%,48% and 30% compared with 19%,24% and 55% after transformation,respectively.

关 键 词：发酵 产氢 细菌 荧光原位杂交 

分 类 号：X172[环境科学与工程—环境科学]