污染食品中氰化物的快速检测方法  

A rapid photoelectric method for on-spot determination of cyanide in contamination food

作　　者：陈超 王红勇[2] 高志贤[2] 

机构地区：[1]总政治部直属工作部卫生局防疫所,北京100000 [2]军事医学科学院卫生学环境医学研究所,天津300050

出　　处：《中国卫生检验杂志》2010年第1期34-35,41,共3页Chinese Journal of Health Laboratory Technology

基　　金："863"计划课题(2007AA06Z419);天津市科技计划项目(092CKFSH02700)

摘　　要：目的：检测试剂和手持式光电比色仪联用的方法,实现对污染食品中氰化物的现场快速定量检测。方法：通过氰化物在碱性条件下,CN-可催化茚三酮自身氧化还原反应,迅速生成红棕色产物（485 nm）,其呈色深浅与氰化物含量成一定相关性,于手持式光电比色仪实现定量测定。结果：氰化物浓度在0.0-0.5 mg/L范围内线性关系良好（r=0.9957） 方法的检出限为0.05 mg/kg、加标回收率为84%-106%。并与国家标准方法检测结果一致。结论：试剂-光电比色仪方法用于氰化物的现场定量检测具有简便、快速、携带方便等特点,值得推广应用。Objective：To establish a rapid and accurate method for the determination of cyanide in contamination food on the spot.Methods：The concentration of cyanide was assayed based on the oxidation-reduction reaction between the cyanide and the ninhydrin in alkaline medium,to form the colored compound（max=485 nm）,and there is a linear relationship between the intensity of color and the concentration of cyanide,so it was mensured by combining with a portable photolometer,and the optimal experimental conditions were also determined.Results：There was good linear relationship between the concentrations of cyanide in the range of 0.0-0.5 mg/L（r=0.9957）,with the detection limit of 0.05 mg/kg.The results obtained by this method were consistent with those by the national standard method.Conclusion：This method combined with photoelectric meter to detect cyanide in food on the spot is a rapid,accurate and convenient method,so it is worth utilizing.

关 键 词：食品 氰化物 快速 测定方法 

分 类 号：R155.5[医药卫生—营养与食品卫生学]