产木聚糖酶芽胞菌Y-12的筛选及产酶条件的研究  

Screening of xylanase producing Bacillus and study on its enzyme production conditions

作　　者：单春乔[1,2] 张琪 赵红岩 刘艳 余丽芸[2] 庄国宏 

机构地区：[1]大连三仪药品有限公司,辽宁大连116036 [2]黑龙江八一农垦大学,黑龙江大庆163319

出　　处：《中国微生态学杂志》2010年第2期118-121,共4页Chinese Journal of Microecology

摘　　要：利用透明圈平板培养和木聚糖发酵试验,筛选出1株高产木聚糖酶的芽胞杆菌,并对该菌株的酶学特性和适宜发酵条件进行研究。结果表明,此菌对秸秆和麦麸的分解率分别为43.3%和63.4%。产酶较高的最适培养条件,即大豆蛋白胨2%、胰蛋白胨3%、酵母粉0.1%和葡萄糖2%。适宜反应温度和pH分别为37℃和7。采用10 L发酵罐发酵,培养48 h后,酶活达3024 U/m l,比三角瓶发酵(酶活达2185 U/m l)提高了38%。A high-xylanase-producing Bacillus strain was screened by transparent zone and xylan assay on the selective medium.Its proper conditions for fermentation and reaction were investigated.Results indicated that the strains could degrade straw and wheat bran,with the degradation rates at 43.3% and 63.4% respectively.On the basis of screening for liquid culture and determination of activity,an optimal culture conditions with higher yield was obtained,which contained 2% of soybean meal,3% of tryptone,0.1% of yeast extract and 2% of glucose;The optimal temperature and pH value was 37 ℃and pH 7 respectively.With 10 L of fermentation tank,the xylanase activity was up to 3024 U/ml after 48 hours of culture,increased by 38% compared with 2185 U/ml by flask liquid fermentation.

关 键 词：芽胞菌 酶活测定 木聚糖酶 发酵条件 

分 类 号：Q939.124[生物学—微生物学] Q556