儿茶素抑制蛋白质类老年色素荧光物质生成活性的研究  

Study on Inhibiting Effects of Tea Catechins on the Formation of Age Pigment Fluorescence Products

作　　者：蔡淑娴[1] 刘仲华[1,2] 黄建安[1] 罗国安[2] 余兴龙[3] 董新荣[1] 杨志辉[1] 叶飞[1] 

机构地区：[1]湖南农业大学茶学教育部重点实验室,湖南长沙410128 [2]清华大学化学系生命有机磷与化学生物学教育部重点实验室,北京100084 [3]湖南农业大学动物医学院,湖南长沙410128

出　　处：《茶叶科学》2010年第1期1-8,共8页Journal of Tea Science

基　　金：国家973项目(2008CB117007);湖南省科技重大专项(NK2007005);农业部茶叶产业技术体系;农业部行业公益专项(NYHYZX07-021-12)

摘　　要：丙二醛(Malondialdehyde,MDA)是机体内普遍存在的活性羰基化合物代表成分之一,能够与蛋白质发生羰-氨交联反应,生成老年色素荧光物质。本文探讨儿茶素单体成分对MDA应激引起的蛋白质类老年色素荧光物质生成体系的影响,并对其构效关系进行初步分析。结果表明:儿茶素能显著抑制MDA诱导的蛋白质类老年色素物质生成,且酯型儿茶素活性较强,抑制作用除与直接清除MDA外,还与其结构的亲核性有关。本研究从一个新的角度揭示了儿茶素抑制羰-氨交联反应是其预防和治疗羰基应激相关疾病的潜在作用机制之一。Malondialdehyde （MDA） is one of the active carbonyl compounds existing in human body, which can react with protein to form age pigment fluorescence products （APFs） by carbonyl-ammonia cross linking reaction. The purpose of this study is to determine whether tea catechins can inhibit the formation of the age pigment fluorescence products induced by MDA-modified human serum albumin （HSA）. Results showed that tea catechins, especially the gallated-catechin, could inhibit the formation of APFs. The structure-activity relationship studies suggested that the inhibiting effects of tea catechins on the formation of APFs were correlated with their nucleophilicity, besides their trapping MDA directly. This research indicated that inhibiting carbonyl-ammonia cross linking reaction may be one of the mechanisms of tea catechins preventing and curing diseases resulting from carbonyl stress reaction.

关 键 词：儿茶素 老年色素荧光物质 羰基应激 羰氨交联产物 

分 类 号：S571.1[农业科学—茶叶生产加工] Q586[农业科学—作物学]