鼠尾胶原在培养鼠下切牙颈环上皮细胞中的应用  

Application of rat tail collagen in the culture of cervical-loop epithelial cells in rat lower incisors

作　　者：胡孝丽[1] 王铎[2] 冷卫东[1] 罗志晓[1] 

机构地区：[1]湖北十堰郧阳医学院附属太和医院口腔科,442000 [2]山东大学口腔医学院牙体科

出　　处：《实用口腔医学杂志》2010年第1期51-54,共4页Journal of Practical Stomatology

基　　金：山东省自然科学基金资助项目(编号:Y2005C64);湖北省十堰市科技局项目(编号:2009s20)

摘　　要：目的:建立用自制鼠尾胶原为贴附底物的大鼠颈环上皮细胞的体外培养模式。方法:制作鼠尾胶原,观察用自制的鼠尾胶原培养出大鼠颈环上皮细胞的效果,并对纯化的上皮细胞进行免疫组织化学染色鉴定。结果:自制的鼠尾胶原能正常地培养出大鼠颈环上皮细胞,呈多角形,克隆状生长,β1整合素和角蛋白免疫组织化学染色阳性。结论:鼠尾胶原可作大鼠颈环上皮细胞的体外培养贴附底物,为今后研究牙齿发育机制提供简便的方法和途径。Objective： To establish a cell culture model of cervical-loop epithelial cells from Wistar rat lower incisors on culture bottle coated with rat tail collagen.Methods： The effect of self-made rat tail collagen on the culture of cervical-loop epithelial cells was observed.The cells were identified by immunohistochemistry staining.Results： Cervical-loop epithelial cells in Wistar rat lower incisors grew well in self-made rat tail collagen.The cervical-loop epithelial cells exhibited positive expression for integrin-β1 and monoclonal antibody CK in immunohistochemistry staining.Conclusion： The cell culture model of cervical-loop epithelial cells in Wistar rat lower incisors with self-made rat tail collagen can be helpful to research tooth development mechanism.

关 键 词：颈环 上皮细胞 细胞培养 鼠尾胶原 

分 类 号：R78[医药卫生—口腔医学]